Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12337 | 5' | -61.5 | NC_003324.1 | + | 45583 | 0.66 | 0.431945 |
Target: 5'- cGGGGUuuUACCUgCGGauGUGCGCGCCuGUa -3' miRNA: 3'- -UCCCGcuGUGGG-GCU--UACGCGCGG-CA- -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 46823 | 0.66 | 0.422797 |
Target: 5'- cGGGGC-ACACCUCG---GCGCcCCGUg -3' miRNA: 3'- -UCCCGcUGUGGGGCuuaCGCGcGGCA- -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 42531 | 0.66 | 0.420075 |
Target: 5'- uGGGaCGACACCgCCGAuucggugacggGUGCucgcuuccugucugGCGCUGg -3' miRNA: 3'- uCCC-GCUGUGG-GGCU-----------UACG--------------CGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 39862 | 0.66 | 0.412869 |
Target: 5'- gAGGGCGagguGCACCgugCCGAgaucaucgagcacGUGC-CGCCGg -3' miRNA: 3'- -UCCCGC----UGUGG---GGCU-------------UACGcGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 8174 | 0.66 | 0.411974 |
Target: 5'- cAGGGCcagucccugcucACugCCCGGcgGCGCGCg-- -3' miRNA: 3'- -UCCCGc-----------UGugGGGCUuaCGCGCGgca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 19125 | 0.66 | 0.404854 |
Target: 5'- uGGGCuGCAaaggCUCGAAUGaCGCGCCu- -3' miRNA: 3'- uCCCGcUGUg---GGGCUUAC-GCGCGGca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 45982 | 0.66 | 0.396065 |
Target: 5'- aAGcGGCGGCGCCgaaUCGAGgaGCGUGCCc- -3' miRNA: 3'- -UC-CCGCUGUGG---GGCUUa-CGCGCGGca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 31733 | 0.66 | 0.387399 |
Target: 5'- -cGGCGGCAacuuCUCCG---GCGCGCCGa -3' miRNA: 3'- ucCCGCUGU----GGGGCuuaCGCGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 14900 | 0.66 | 0.37886 |
Target: 5'- gAGGGCGACGCgCUCGAc-GCGgucaacaacaUGCCGg -3' miRNA: 3'- -UCCCGCUGUG-GGGCUuaCGC----------GCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 30123 | 0.66 | 0.37886 |
Target: 5'- aAGGGuCGACGCgCUGGAUaGCuGCGuuGUc -3' miRNA: 3'- -UCCC-GCUGUGgGGCUUA-CG-CGCggCA- -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 37083 | 0.66 | 0.37886 |
Target: 5'- gAGGGCGA-ACCgCUuuAUGCGCGCUa- -3' miRNA: 3'- -UCCCGCUgUGG-GGcuUACGCGCGGca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 26654 | 0.67 | 0.359703 |
Target: 5'- gAGGGUGACACCugccugcagaauuuCCGGAucgaUGC-CGCUGUu -3' miRNA: 3'- -UCCCGCUGUGG--------------GGCUU----ACGcGCGGCA- -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 48975 | 0.67 | 0.354009 |
Target: 5'- cGGGUGGCGgagUCCUGGAUGCGgGCg-- -3' miRNA: 3'- uCCCGCUGU---GGGGCUUACGCgCGgca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 50070 | 0.67 | 0.353201 |
Target: 5'- uGGGCGGCgaucauuGCCCCcaucaggGCaGCGCCGa -3' miRNA: 3'- uCCCGCUG-------UGGGGcuua---CG-CGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 7281 | 0.67 | 0.330331 |
Target: 5'- cGGGGUgucaaaggGACGCCaUCGAGcucgGCGCGCUGg -3' miRNA: 3'- -UCCCG--------CUGUGG-GGCUUa---CGCGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 17693 | 0.67 | 0.330331 |
Target: 5'- -cGGCGGCAUCgUGcucaAcgGCGCGCCGg -3' miRNA: 3'- ucCCGCUGUGGgGC----UuaCGCGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 56549 | 0.68 | 0.315206 |
Target: 5'- cGGGCGAaguuGCCCgCGAcccAUGC-CGCCGc -3' miRNA: 3'- uCCCGCUg---UGGG-GCU---UACGcGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 42284 | 0.68 | 0.315206 |
Target: 5'- cGGGuCGGCaACgCUGAGUGCGcCGCCu- -3' miRNA: 3'- uCCC-GCUG-UGgGGCUUACGC-GCGGca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 32898 | 0.68 | 0.315206 |
Target: 5'- cGaGGCGGCugCCCGcca-UGCGCCGa -3' miRNA: 3'- uC-CCGCUGugGGGCuuacGCGCGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 15649 | 0.68 | 0.30061 |
Target: 5'- -uGGCGACgGCCCCG---GCGCGCuCGa -3' miRNA: 3'- ucCCGCUG-UGGGGCuuaCGCGCG-GCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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