Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12337 | 5' | -61.5 | NC_003324.1 | + | 14495 | 0.7 | 0.241386 |
Target: 5'- cGGGCGugAUCCCcaucGAGgcguuUGCGCGCuCGg -3' miRNA: 3'- uCCCGCugUGGGG----CUU-----ACGCGCG-GCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 34152 | 0.71 | 0.197211 |
Target: 5'- cAGGGCGGaGCCgCUGGcauUGgGCGCCGUg -3' miRNA: 3'- -UCCCGCUgUGG-GGCUu--ACgCGCGGCA- -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 7436 | 0.71 | 0.187328 |
Target: 5'- aAGGGUGcuGCugCCCGAGUGCuGCuGCUGc -3' miRNA: 3'- -UCCCGC--UGugGGGCUUACG-CG-CGGCa -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 30456 | 0.72 | 0.168864 |
Target: 5'- gAGGGCGGCAUggCCGAggGCGaCGCCa- -3' miRNA: 3'- -UCCCGCUGUGg-GGCUuaCGC-GCGGca -5' |
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12337 | 5' | -61.5 | NC_003324.1 | + | 17902 | 1.07 | 0.000379 |
Target: 5'- aAGGGCGACACCCCGAAUGCGCGCCGUg -3' miRNA: 3'- -UCCCGCUGUGGGGCUUACGCGCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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