miRNA: 3'- -UC-CCGCUGUGG---GGCUUa-CGCGCGGca -5'
miRNA: 3'- -UCCCGcUGUGGGGCuuaCGCGcGGCA- -5'
miRNA: 3'- uCCCGCUGU---GGGGCUUACGCgCGgca -5'
miRNA: 3'- uCCCGCUG-------UGGGGcuua---CG-CGCGGCa -5'
miRNA: 3'- uCCCGCUg---UGGG-GCU---UACGcGCGGCa -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12337 | 5' | -61.5 | NC_003324.1 | + | 45982 | 0.66 | 0.396065 |
|
Target: 5'- aAGcGGCGGCGCCgaaUCGAGgaGCGUGCCc- -3' miRNA: 3'- -UC-CCGCUGUGG---GGCUUa-CGCGCGGca -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 46823 | 0.66 | 0.422797 |
|
Target: 5'- cGGGGC-ACACCUCG---GCGCcCCGUg -3' miRNA: 3'- -UCCCGcUGUGGGGCuuaCGCGcGGCA- -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 48975 | 0.67 | 0.354009 |
|
Target: 5'- cGGGUGGCGgagUCCUGGAUGCGgGCg-- -3' miRNA: 3'- uCCCGCUGU---GGGGCUUACGCgCGgca -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 50070 | 0.67 | 0.353201 |
|
Target: 5'- uGGGCGGCgaucauuGCCCCcaucaggGCaGCGCCGa -3' miRNA: 3'- uCCCGCUG-------UGGGGcuua---CG-CGCGGCa -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 56549 | 0.68 | 0.315206 |
|
Target: 5'- cGGGCGAaguuGCCCgCGAcccAUGC-CGCCGc -3' miRNA: 3'- uCCCGCUg---UGGG-GCU---UACGcGCGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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