Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12337 | 5' | -61.5 | NC_003324.1 | + | 14900 | 0.66 | 0.37886 |
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Target: 5'- gAGGGCGACGCgCUCGAc-GCGgucaacaacaUGCCGg -3' miRNA: 3'- -UCCCGCUGUG-GGGCUuaCGC----------GCGGCa -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 14495 | 0.7 | 0.241386 |
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Target: 5'- cGGGCGugAUCCCcaucGAGgcguuUGCGCGCuCGg -3' miRNA: 3'- uCCCGCugUGGGG----CUU-----ACGCGCG-GCa -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 8174 | 0.66 | 0.411974 |
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Target: 5'- cAGGGCcagucccugcucACugCCCGGcgGCGCGCg-- -3' miRNA: 3'- -UCCCGc-----------UGugGGGCUuaCGCGCGgca -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 7436 | 0.71 | 0.187328 |
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Target: 5'- aAGGGUGcuGCugCCCGAGUGCuGCuGCUGc -3' miRNA: 3'- -UCCCGC--UGugGGGCUUACG-CG-CGGCa -5' |
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| 12337 | 5' | -61.5 | NC_003324.1 | + | 7281 | 0.67 | 0.330331 |
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Target: 5'- cGGGGUgucaaaggGACGCCaUCGAGcucgGCGCGCUGg -3' miRNA: 3'- -UCCCG--------CUGUGG-GGCUUa---CGCGCGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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