Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12341 | 5' | -53.2 | NC_003324.1 | + | 24556 | 0.69 | 0.663602 |
Target: 5'- -aGAGCAGuGCCgGAAGAucgucaccGCUgcGGGCGACc -3' miRNA: 3'- agUUCGUC-CGG-CUUCU--------UGA--CCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 55033 | 0.69 | 0.652422 |
Target: 5'- cCGGGCAGGCaaggCGGAcGGCUGGgcugccGCGACg -3' miRNA: 3'- aGUUCGUCCG----GCUUcUUGACC------UGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 15470 | 0.69 | 0.652422 |
Target: 5'- -gAGGCcaAGGCCGAGGAuagcGACGACu -3' miRNA: 3'- agUUCG--UCCGGCUUCUugacCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 29876 | 0.69 | 0.627773 |
Target: 5'- aUCAGGCAGGgUGGAuacggcgcgaucuucGGcgcgcugcuggccauGCUGGACGGCg -3' miRNA: 3'- -AGUUCGUCCgGCUU---------------CU---------------UGACCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 28523 | 0.69 | 0.618809 |
Target: 5'- --cGGCAacGGCUGggGAACgacaGACGACa -3' miRNA: 3'- aguUCGU--CCGGCuuCUUGac--CUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 16439 | 0.7 | 0.596443 |
Target: 5'- gCGAGCAGGUC------CUGGACGACg -3' miRNA: 3'- aGUUCGUCCGGcuucuuGACCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 49355 | 0.7 | 0.585302 |
Target: 5'- cCAacAGCGGuGUCGAcGAuCUGGGCGACc -3' miRNA: 3'- aGU--UCGUC-CGGCUuCUuGACCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 18591 | 0.7 | 0.596443 |
Target: 5'- uUUAGGCGGGCCau----CUGGugGGCg -3' miRNA: 3'- -AGUUCGUCCGGcuucuuGACCugCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 21762 | 0.71 | 0.552158 |
Target: 5'- cUCu-GCAGGgCGAGGAACUcguccGGCGACa -3' miRNA: 3'- -AGuuCGUCCgGCUUCUUGAc----CUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 30801 | 0.73 | 0.417592 |
Target: 5'- gCAGGC-GGCCGAcgccaAGAAUUGGugGgACa -3' miRNA: 3'- aGUUCGuCCGGCU-----UCUUGACCugC-UG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 19323 | 1.11 | 0.001147 |
Target: 5'- cUCAAGCAGGCCGAAGAACUGGACGACa -3' miRNA: 3'- -AGUUCGUCCGGCUUCUUGACCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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