Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12341 | 5' | -53.2 | NC_003324.1 | + | 42551 | 0.67 | 0.781996 |
Target: 5'- gCAAGCucaAGGUgaAAGAAUgGGACGACa -3' miRNA: 3'- aGUUCG---UCCGgcUUCUUGaCCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 30491 | 0.66 | 0.791036 |
Target: 5'- cCGAGCAGGCCcucGGAaACUGcgccugcgaccguGGCGACc -3' miRNA: 3'- aGUUCGUCCGGcu-UCU-UGAC-------------CUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 11589 | 0.66 | 0.792032 |
Target: 5'- ---cGCGGcuccuGCCGGAG-ACUGGuGCGACg -3' miRNA: 3'- aguuCGUC-----CGGCUUCuUGACC-UGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 17099 | 0.66 | 0.795998 |
Target: 5'- aUCAAgacGCGGGCC-AAGAACgcagcgaaacgugaGGAUGGCg -3' miRNA: 3'- -AGUU---CGUCCGGcUUCUUGa-------------CCUGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 35455 | 0.66 | 0.801894 |
Target: 5'- cCGAGCAGGCUGcAGcAGCacaGGcCGACc -3' miRNA: 3'- aGUUCGUCCGGCuUC-UUGa--CCuGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 32166 | 0.66 | 0.801894 |
Target: 5'- aCGAGCcgaggcgcagcgAGGCCGAAGAGCcauuUGACg -3' miRNA: 3'- aGUUCG------------UCCGGCUUCUUGaccuGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 2730 | 0.66 | 0.820116 |
Target: 5'- -aGGGCaAGGUCGAugacguuuuGGGACUGGaucaacaGCGGCa -3' miRNA: 3'- agUUCG-UCCGGCU---------UCUUGACC-------UGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 34856 | 0.66 | 0.821055 |
Target: 5'- aUCGAGCAGGCCcuugacgauuuGAAGGAUcagcGGACc-- -3' miRNA: 3'- -AGUUCGUCCGG-----------CUUCUUGa---CCUGcug -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 39784 | 0.66 | 0.830332 |
Target: 5'- ---cGCAGGCCGGGcacaUGGcGCGACg -3' miRNA: 3'- aguuCGUCCGGCUUcuugACC-UGCUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 49347 | 0.66 | 0.830332 |
Target: 5'- ---cGCAGG-CGAGGAuCUGGACacgaaGACg -3' miRNA: 3'- aguuCGUCCgGCUUCUuGACCUG-----CUG- -5' |
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12341 | 5' | -53.2 | NC_003324.1 | + | 7995 | 0.66 | 0.833983 |
Target: 5'- -uGAGCGGGCCGgcGAACagaaaguuuccuccGGuCGAUg -3' miRNA: 3'- agUUCGUCCGGCuuCUUGa-------------CCuGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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