Results 21 - 35 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12344 | 3' | -54.8 | NC_003324.1 | + | 49906 | 0.68 | 0.627341 |
Target: 5'- cGGCGCGCUCgUGCUCGccuUCGCuGCc- -3' miRNA: 3'- -CCGUGCGGGaGCGAGUu--AGCGuUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 26198 | 0.68 | 0.627341 |
Target: 5'- gGGCuGCGucugcgucgucCCCUCGCUCGGcaGCAGCAa -3' miRNA: 3'- -CCG-UGC-----------GGGAGCGAGUUagCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 57245 | 0.68 | 0.615107 |
Target: 5'- cGGCAgGCCCUUGgcuucgcaCUCAGUCGggggcguCAGCAg -3' miRNA: 3'- -CCGUgCGGGAGC--------GAGUUAGC-------GUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 48936 | 0.69 | 0.549012 |
Target: 5'- uGGUcaAgGCCgUCGCUucuucggCAGUCGCGGCAUa -3' miRNA: 3'- -CCG--UgCGGgAGCGA-------GUUAGCGUUGUA- -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 53895 | 0.69 | 0.539263 |
Target: 5'- uGGUGCGCCC-CGCcgCAAgaucaucagCGCGGCAa -3' miRNA: 3'- -CCGUGCGGGaGCGa-GUUa--------GCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 36446 | 0.69 | 0.539263 |
Target: 5'- gGGCAUGCCgUCGCgcc--CGCGGCGc -3' miRNA: 3'- -CCGUGCGGgAGCGaguuaGCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 4 | 0.7 | 0.476023 |
Target: 5'- uGGCAUGUCCUCgaccugguGCUCAGUCcCAACc- -3' miRNA: 3'- -CCGUGCGGGAG--------CGAGUUAGcGUUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 36511 | 0.71 | 0.407245 |
Target: 5'- cGGCAUGCCCUacgGCaUGAUCcGCAACAUc -3' miRNA: 3'- -CCGUGCGGGAg--CGaGUUAG-CGUUGUA- -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 40607 | 0.71 | 0.426266 |
Target: 5'- aGGCuucuCGCCg-CGCUCGAUCGUGGCu- -3' miRNA: 3'- -CCGu---GCGGgaGCGAGUUAGCGUUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 5184 | 0.71 | 0.444817 |
Target: 5'- aGGCAucauUGCCCUCGCUUAccccucuAUCGCuGGCGc -3' miRNA: 3'- -CCGU----GCGGGAGCGAGU-------UAGCG-UUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 55813 | 0.71 | 0.416689 |
Target: 5'- cGGCGUGCCCUCGUggauuUUGCGGCAg -3' miRNA: 3'- -CCGUGCGGGAGCGaguu-AGCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 29007 | 0.72 | 0.379733 |
Target: 5'- cGGCACGCUCaaaGCaggagccggaaUCGGUCGCAGCGa -3' miRNA: 3'- -CCGUGCGGGag-CG-----------AGUUAGCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 7760 | 0.73 | 0.336726 |
Target: 5'- aGCACG-CCUCGCagggaggcgCGAUCGCAACGg -3' miRNA: 3'- cCGUGCgGGAGCGa--------GUUAGCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 56204 | 0.75 | 0.254991 |
Target: 5'- cGGCAgccuCGCCUaUCGCUCGAUCgGCGGCGa -3' miRNA: 3'- -CCGU----GCGGG-AGCGAGUUAG-CGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 20582 | 1.07 | 0.001391 |
Target: 5'- gGGCACGCCCUCGCUCAAUCGCAACAUc -3' miRNA: 3'- -CCGUGCGGGAGCGAGUUAGCGUUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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