Results 21 - 35 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12344 | 3' | -54.8 | NC_003324.1 | + | 26198 | 0.68 | 0.627341 |
Target: 5'- gGGCuGCGucugcgucgucCCCUCGCUCGGcaGCAGCAa -3' miRNA: 3'- -CCG-UGC-----------GGGAGCGAGUUagCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 49906 | 0.68 | 0.627341 |
Target: 5'- cGGCGCGCUCgUGCUCGccuUCGCuGCc- -3' miRNA: 3'- -CCGUGCGGGaGCGAGUu--AGCGuUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 20455 | 0.67 | 0.660696 |
Target: 5'- cGGC-CGUCauugagaacgCGCUCGAUCGCcGCAUc -3' miRNA: 3'- -CCGuGCGGga--------GCGAGUUAGCGuUGUA- -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 57245 | 0.68 | 0.615107 |
Target: 5'- cGGCAgGCCCUUGgcuucgcaCUCAGUCGggggcguCAGCAg -3' miRNA: 3'- -CCGUgCGGGAGC--------GAGUUAGC-------GUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 40607 | 0.71 | 0.426266 |
Target: 5'- aGGCuucuCGCCg-CGCUCGAUCGUGGCu- -3' miRNA: 3'- -CCGu---GCGGgaGCGAGUUAGCGUUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 36511 | 0.71 | 0.407245 |
Target: 5'- cGGCAUGCCCUacgGCaUGAUCcGCAACAUc -3' miRNA: 3'- -CCGUGCGGGAg--CGaGUUAG-CGUUGUA- -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 29007 | 0.72 | 0.379733 |
Target: 5'- cGGCACGCUCaaaGCaggagccggaaUCGGUCGCAGCGa -3' miRNA: 3'- -CCGUGCGGGag-CG-----------AGUUAGCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 54703 | 0.67 | 0.654036 |
Target: 5'- cGCACGUCCUCgagucuuucgaucuuGCUCGAUCG--ACGUa -3' miRNA: 3'- cCGUGCGGGAG---------------CGAGUUAGCguUGUA- -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 54314 | 0.67 | 0.682814 |
Target: 5'- cGGUgccGCGCCUUCGCUUuga-GCAGCc- -3' miRNA: 3'- -CCG---UGCGGGAGCGAGuuagCGUUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 42260 | 0.67 | 0.682814 |
Target: 5'- cGGCGCaguGCCgUCGUUCGgcaaguacgAUCGCGGCc- -3' miRNA: 3'- -CCGUG---CGGgAGCGAGU---------UAGCGUUGua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 37510 | 0.66 | 0.704735 |
Target: 5'- uGCGCGgCaaugCGCUCGAUaGCAACAa -3' miRNA: 3'- cCGUGCgGga--GCGAGUUAgCGUUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 26798 | 0.66 | 0.715592 |
Target: 5'- aGGCccGCGCCCU-GCUgcGUCGCAAg-- -3' miRNA: 3'- -CCG--UGCGGGAgCGAguUAGCGUUgua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 35941 | 0.66 | 0.737042 |
Target: 5'- uGGCGCGCCacuguUCGC-CAggCGCAGg-- -3' miRNA: 3'- -CCGUGCGGg----AGCGaGUuaGCGUUgua -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 6503 | 0.66 | 0.747611 |
Target: 5'- uGCGUGCCCUUcacgaGCUgGAUCGCuGCAg -3' miRNA: 3'- cCGUGCGGGAG-----CGAgUUAGCGuUGUa -5' |
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12344 | 3' | -54.8 | NC_003324.1 | + | 20582 | 1.07 | 0.001391 |
Target: 5'- gGGCACGCCCUCGCUCAAUCGCAACAUc -3' miRNA: 3'- -CCGUGCGGGAGCGAGUUAGCGUUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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