Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12345 | 5' | -61.5 | NC_003324.1 | + | 55132 | 0.7 | 0.250664 |
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Target: 5'- --cUGCGCCGUcGCGgcagcccaGCCGUCCG-CCu -3' miRNA: 3'- gcuACGCGGCAcCGC--------CGGCAGGCuGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 24832 | 0.7 | 0.250664 |
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Target: 5'- gGAUcGCGCCGacaaGGuCGGCCGUCUuGGCa -3' miRNA: 3'- gCUA-CGCGGCa---CC-GCCGGCAGG-CUGg -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 31621 | 0.71 | 0.210867 |
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Target: 5'- gCGG-GCGCgCGUGGCGGCCauucUCaCGACg -3' miRNA: 3'- -GCUaCGCG-GCACCGCCGGc---AG-GCUGg -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 23752 | 0.72 | 0.20257 |
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Target: 5'- gCGAcguUGCGCCGgaaaucgggguccgaUucGGCGGCgCGUUCGGCCu -3' miRNA: 3'- -GCU---ACGCGGC---------------A--CCGCCG-GCAGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 12123 | 0.72 | 0.20054 |
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Target: 5'- gCGcUGCGUCGUGaUGGaCCGUCCGcCCg -3' miRNA: 3'- -GCuACGCGGCACcGCC-GGCAGGCuGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 29226 | 0.73 | 0.159366 |
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Target: 5'- aCGGUGCGCUcaccaagacGGCGGCUGUUCgGGCCu -3' miRNA: 3'- -GCUACGCGGca-------CCGCCGGCAGG-CUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 14600 | 0.73 | 0.151316 |
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Target: 5'- gGcgGCGCaggcgguagCGUGGCGGCCGgagcuaCGGCCc -3' miRNA: 3'- gCuaCGCG---------GCACCGCCGGCag----GCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 21602 | 1.1 | 0.000263 |
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Target: 5'- uCGAUGCGCCGUGGCGGCCGUCCGACCu -3' miRNA: 3'- -GCUACGCGGCACCGCCGGCAGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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