Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12345 | 5' | -61.5 | NC_003324.1 | + | 20590 | 0.66 | 0.451862 |
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Target: 5'- gGAUccgGUGCUGUGGC-GCCGUC--GCCa -3' miRNA: 3'- gCUA---CGCGGCACCGcCGGCAGgcUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 16899 | 0.66 | 0.415516 |
|
Target: 5'- -uGUGCGCCGauuugcaaggUGGCGGCUG-CCaGCg -3' miRNA: 3'- gcUACGCGGC----------ACCGCCGGCaGGcUGg -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 16214 | 0.66 | 0.415516 |
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Target: 5'- uCGAUaccGCGaCGgGGCaGGCCGUgCCGACg -3' miRNA: 3'- -GCUA---CGCgGCaCCG-CCGGCA-GGCUGg -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 14600 | 0.73 | 0.151316 |
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Target: 5'- gGcgGCGCaggcgguagCGUGGCGGCCGgagcuaCGGCCc -3' miRNA: 3'- gCuaCGCG---------GCACCGCCGGCag----GCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 13173 | 0.68 | 0.331455 |
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Target: 5'- gCGAggcUGCGUCGgcggcggucggcGGCGGCgGUaCCGGCUg -3' miRNA: 3'- -GCU---ACGCGGCa-----------CCGCCGgCA-GGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 13066 | 0.66 | 0.470675 |
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Target: 5'- aCGAuuUGCaGCCGguaccGCcGCCG-CCGACCg -3' miRNA: 3'- -GCU--ACG-CGGCac---CGcCGGCaGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 12123 | 0.72 | 0.20054 |
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Target: 5'- gCGcUGCGUCGUGaUGGaCCGUCCGcCCg -3' miRNA: 3'- -GCuACGCGGCACcGCC-GGCAGGCuGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 8482 | 0.66 | 0.415516 |
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Target: 5'- ---cGCcCCGaUGGUGGCCGgagCUGAUCg -3' miRNA: 3'- gcuaCGcGGC-ACCGCCGGCa--GGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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