Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12347 | 5' | -57.6 | NC_003324.1 | + | 22686 | 1.06 | 0.000771 |
Target: 5'- gCUCGGCAACGCGGCAGGCAGCCAAAAg -3' miRNA: 3'- -GAGCCGUUGCGCCGUCCGUCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 14598 | 0.76 | 0.129532 |
Target: 5'- -cCGGCGGCGCaGGCGguagcguGGCGGCCGGAGc -3' miRNA: 3'- gaGCCGUUGCG-CCGU-------CCGUCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 42380 | 0.76 | 0.126306 |
Target: 5'- -cCGGC-ACGUGGCAGGCGGCgCAc-- -3' miRNA: 3'- gaGCCGuUGCGCCGUCCGUCG-GUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 21375 | 0.76 | 0.133579 |
Target: 5'- -cCGGCGAgGCGGCGaacGCGGCCGAGAu -3' miRNA: 3'- gaGCCGUUgCGCCGUc--CGUCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 44091 | 0.75 | 0.153475 |
Target: 5'- gCUCGGCAugauCcCGGC-GGCAGCCGAGGa -3' miRNA: 3'- -GAGCCGUu---GcGCCGuCCGUCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 32816 | 0.74 | 0.180871 |
Target: 5'- uUCGGC-GCaUGGCGGGCAGCCGc-- -3' miRNA: 3'- gAGCCGuUGcGCCGUCCGUCGGUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 8870 | 0.72 | 0.224073 |
Target: 5'- uUCGGUAGCcGCGGgAGauGCGGCCAAAu -3' miRNA: 3'- gAGCCGUUG-CGCCgUC--CGUCGGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 16213 | 0.7 | 0.312617 |
Target: 5'- gUCGauaccGCGACGgGGCAGGCcguGCCGAc- -3' miRNA: 3'- gAGC-----CGUUGCgCCGUCCGu--CGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 53220 | 0.7 | 0.328385 |
Target: 5'- cCUCGGaUGAUGCGGCccuuacggucGGGgAGCCAGAc -3' miRNA: 3'- -GAGCC-GUUGCGCCG----------UCCgUCGGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 31803 | 0.69 | 0.361658 |
Target: 5'- uUCGGCAACGuCGGCG---AGCCAAGc -3' miRNA: 3'- gAGCCGUUGC-GCCGUccgUCGGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 44712 | 0.69 | 0.370335 |
Target: 5'- -cCGGCAuCGCaugGGCAGuCAGCCAAGg -3' miRNA: 3'- gaGCCGUuGCG---CCGUCcGUCGGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 5497 | 0.69 | 0.370335 |
Target: 5'- aCUCGGCGcCGCagguGCGGGCgccaucuuccgAGCCGAAAu -3' miRNA: 3'- -GAGCCGUuGCGc---CGUCCG-----------UCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 49051 | 0.68 | 0.43492 |
Target: 5'- -gCGGCGGCGgGGCAauccauccGGCucugcaccggGGCCGAAAg -3' miRNA: 3'- gaGCCGUUGCgCCGU--------CCG----------UCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 32435 | 0.68 | 0.405507 |
Target: 5'- -aCGGUucuugauGACGCuGGCAacGGCAGCCAu-- -3' miRNA: 3'- gaGCCG-------UUGCG-CCGU--CCGUCGGUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 13163 | 0.68 | 0.406436 |
Target: 5'- gUCGGCGGCGguCGGC-GGCGGCgGu-- -3' miRNA: 3'- gAGCCGUUGC--GCCGuCCGUCGgUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 320 | 0.68 | 0.444669 |
Target: 5'- -cCGGCGAUGCGGCcGcCAGCCc--- -3' miRNA: 3'- gaGCCGUUGCGCCGuCcGUCGGuuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 24856 | 0.68 | 0.425296 |
Target: 5'- uCUUGGCAAucUGCucGCGGGCGGCCu--- -3' miRNA: 3'- -GAGCCGUU--GCGc-CGUCCGUCGGuuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 44308 | 0.68 | 0.444669 |
Target: 5'- -cCGG--GCGCGGUGGcGCGGCCGAu- -3' miRNA: 3'- gaGCCguUGCGCCGUC-CGUCGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 145 | 0.67 | 0.484835 |
Target: 5'- -cCGGCGcauaagagcgugGCuGCGGCAGGCAGaCGGGu -3' miRNA: 3'- gaGCCGU------------UG-CGCCGUCCGUCgGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 45423 | 0.67 | 0.464526 |
Target: 5'- -aCGGCugUGauCGGCGGuGCAGCCAGu- -3' miRNA: 3'- gaGCCGuuGC--GCCGUC-CGUCGGUUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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