Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12347 | 5' | -57.6 | NC_003324.1 | + | 32536 | 0.66 | 0.558884 |
Target: 5'- --aGGCGACGagGGCAuGGCuGCCGu-- -3' miRNA: 3'- gagCCGUUGCg-CCGU-CCGuCGGUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 36875 | 0.66 | 0.558884 |
Target: 5'- -aCGGCGACGaGGCAauGGaugAGCCGGAGc -3' miRNA: 3'- gaGCCGUUGCgCCGU--CCg--UCGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 29958 | 0.66 | 0.558884 |
Target: 5'- --gGGCGuccugcguaGCGGC-GGCAGCCGGGu -3' miRNA: 3'- gagCCGUug-------CGCCGuCCGUCGGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 3511 | 0.66 | 0.557799 |
Target: 5'- -aCGGUGcuuucccACGCGGaAGuGCAGCCAAAu -3' miRNA: 3'- gaGCCGU-------UGCGCCgUC-CGUCGGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 6099 | 0.66 | 0.548072 |
Target: 5'- gUCGGCAGgaGCGGacuGGGCGGCa---- -3' miRNA: 3'- gAGCCGUUg-CGCCg--UCCGUCGguuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 41657 | 0.66 | 0.548072 |
Target: 5'- gUCGGCGGCGCGac--GUAGCCGAc- -3' miRNA: 3'- gAGCCGUUGCGCcgucCGUCGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 44917 | 0.66 | 0.537326 |
Target: 5'- -aCGGCA--GCaGCGGGCAcGCCAAu- -3' miRNA: 3'- gaGCCGUugCGcCGUCCGU-CGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 36288 | 0.66 | 0.537326 |
Target: 5'- -aUGGCAuCGgGGCGGGCGauuucuuccGCCAGc- -3' miRNA: 3'- gaGCCGUuGCgCCGUCCGU---------CGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 16169 | 0.66 | 0.526655 |
Target: 5'- -cUGGCAcCGaGGCGGGCAccgcagcucccGCCAGAAa -3' miRNA: 3'- gaGCCGUuGCgCCGUCCGU-----------CGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 43324 | 0.66 | 0.526655 |
Target: 5'- --aGGCGACcCuGCAGGCGGCCc--- -3' miRNA: 3'- gagCCGUUGcGcCGUCCGUCGGuuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 23546 | 0.66 | 0.516064 |
Target: 5'- -aCaGCGugGCGGCGGGCGcGCUc--- -3' miRNA: 3'- gaGcCGUugCGCCGUCCGU-CGGuuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 19618 | 0.67 | 0.50556 |
Target: 5'- uUUGaGCAGCGCGggcuGCAGGuCAGUCAGu- -3' miRNA: 3'- gAGC-CGUUGCGC----CGUCC-GUCGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 14819 | 0.67 | 0.494112 |
Target: 5'- aUCGGCAGCuuccuggGCGGCAucGGCGGaCUGAu- -3' miRNA: 3'- gAGCCGUUG-------CGCCGU--CCGUC-GGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 31639 | 0.67 | 0.492044 |
Target: 5'- cCUUGGCAuaucgagaccgcggGCGCGcGU-GGCGGCCAu-- -3' miRNA: 3'- -GAGCCGU--------------UGCGC-CGuCCGUCGGUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 46322 | 0.67 | 0.484835 |
Target: 5'- gCUCGcG-AGCGCGGUGGGCucuGCCGu-- -3' miRNA: 3'- -GAGC-CgUUGCGCCGUCCGu--CGGUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 145 | 0.67 | 0.484835 |
Target: 5'- -cCGGCGcauaagagcgugGCuGCGGCAGGCAGaCGGGu -3' miRNA: 3'- gaGCCGU------------UG-CGCCGUCCGUCgGUUUu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 16996 | 0.67 | 0.484835 |
Target: 5'- aUCGGUGACGCuGGCAG-CcGCCAc-- -3' miRNA: 3'- gAGCCGUUGCG-CCGUCcGuCGGUuuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 43645 | 0.67 | 0.474626 |
Target: 5'- aUCGGCugacguuGCGCGGCGuguGGCugauGCUGGAAg -3' miRNA: 3'- gAGCCGu------UGCGCCGU---CCGu---CGGUUUU- -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 45423 | 0.67 | 0.464526 |
Target: 5'- -aCGGCugUGauCGGCGGuGCAGCCAGu- -3' miRNA: 3'- gaGCCGuuGC--GCCGUC-CGUCGGUUuu -5' |
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12347 | 5' | -57.6 | NC_003324.1 | + | 11554 | 0.67 | 0.464526 |
Target: 5'- uUCGGCcuuuGCG-GGCGaGCAGCCGAu- -3' miRNA: 3'- gAGCCGu---UGCgCCGUcCGUCGGUUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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