miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12348 5' -56.6 NC_003324.1 + 23280 1.06 0.000931
Target:  5'- cAUCUGGCGCACGAUGCAGAGCGGUACc -3'
miRNA:   3'- -UAGACCGCGUGCUACGUCUCGCCAUG- -5'
12348 5' -56.6 NC_003324.1 + 4396 0.75 0.170919
Target:  5'- gAUCUGGCGCAUGuuGUGC---GCGGUGCg -3'
miRNA:   3'- -UAGACCGCGUGC--UACGucuCGCCAUG- -5'
12348 5' -56.6 NC_003324.1 + 4186 0.73 0.229814
Target:  5'- gGUgaGGCGCugGAUGCAGAaaguaucauCGGUGCc -3'
miRNA:   3'- -UAgaCCGCGugCUACGUCUc--------GCCAUG- -5'
12348 5' -56.6 NC_003324.1 + 48325 0.71 0.320487
Target:  5'- --gUGuGUGCugGAUGUGGaAGCGGUACc -3'
miRNA:   3'- uagAC-CGCGugCUACGUC-UCGCCAUG- -5'
12348 5' -56.6 NC_003324.1 + 35580 0.7 0.388436
Target:  5'- aGUC-GGCGCugGAaGCAGc-CGGUACg -3'
miRNA:   3'- -UAGaCCGCGugCUaCGUCucGCCAUG- -5'
12348 5' -56.6 NC_003324.1 + 48036 0.69 0.415297
Target:  5'- gGUCUGGaugucucCGCuaugGAUGCAGAGCGG-ACc -3'
miRNA:   3'- -UAGACC-------GCGug--CUACGUCUCGCCaUG- -5'
12348 5' -56.6 NC_003324.1 + 19737 0.69 0.425779
Target:  5'- aAUCUGGCGCGCuuguuucaGCAGGGcCGGUc- -3'
miRNA:   3'- -UAGACCGCGUGcua-----CGUCUC-GCCAug -5'
12348 5' -56.6 NC_003324.1 + 28811 0.68 0.475322
Target:  5'- ---cGGCGCGgGAacUGCAGAGCaGGUuucgGCg -3'
miRNA:   3'- uagaCCGCGUgCU--ACGUCUCG-CCA----UG- -5'
12348 5' -56.6 NC_003324.1 + 7840 0.67 0.549082
Target:  5'- cUCUGGCuuGCGCaGAaugGCGGGGCGGc-- -3'
miRNA:   3'- uAGACCG--CGUG-CUa--CGUCUCGCCaug -5'
12348 5' -56.6 NC_003324.1 + 8741 0.67 0.549082
Target:  5'- gAUCuUGGCG-GCGGUGuCGGAGaaguCGGUGCg -3'
miRNA:   3'- -UAG-ACCGCgUGCUAC-GUCUC----GCCAUG- -5'
12348 5' -56.6 NC_003324.1 + 42491 0.66 0.602765
Target:  5'- uGUCUGGCGCugGcgcuguUGCGGAcccGCGaccaggauaccgcGUACg -3'
miRNA:   3'- -UAGACCGCGugCu-----ACGUCU---CGC-------------CAUG- -5'
12348 5' -56.6 NC_003324.1 + 30011 0.66 0.603871
Target:  5'- cUCUGGCGaCGCGAUGCu--GCGccACc -3'
miRNA:   3'- uAGACCGC-GUGCUACGucuCGCcaUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.