Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12348 | 5' | -56.6 | NC_003324.1 | + | 4186 | 0.73 | 0.229814 |
Target: 5'- gGUgaGGCGCugGAUGCAGAaaguaucauCGGUGCc -3' miRNA: 3'- -UAgaCCGCGugCUACGUCUc--------GCCAUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 4396 | 0.75 | 0.170919 |
Target: 5'- gAUCUGGCGCAUGuuGUGC---GCGGUGCg -3' miRNA: 3'- -UAGACCGCGUGC--UACGucuCGCCAUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 7840 | 0.67 | 0.549082 |
Target: 5'- cUCUGGCuuGCGCaGAaugGCGGGGCGGc-- -3' miRNA: 3'- uAGACCG--CGUG-CUa--CGUCUCGCCaug -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 8741 | 0.67 | 0.549082 |
Target: 5'- gAUCuUGGCG-GCGGUGuCGGAGaaguCGGUGCg -3' miRNA: 3'- -UAG-ACCGCgUGCUAC-GUCUC----GCCAUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 19737 | 0.69 | 0.425779 |
Target: 5'- aAUCUGGCGCGCuuguuucaGCAGGGcCGGUc- -3' miRNA: 3'- -UAGACCGCGUGcua-----CGUCUC-GCCAug -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 23280 | 1.06 | 0.000931 |
Target: 5'- cAUCUGGCGCACGAUGCAGAGCGGUACc -3' miRNA: 3'- -UAGACCGCGUGCUACGUCUCGCCAUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 28811 | 0.68 | 0.475322 |
Target: 5'- ---cGGCGCGgGAacUGCAGAGCaGGUuucgGCg -3' miRNA: 3'- uagaCCGCGUgCU--ACGUCUCG-CCA----UG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 30011 | 0.66 | 0.603871 |
Target: 5'- cUCUGGCGaCGCGAUGCu--GCGccACc -3' miRNA: 3'- uAGACCGC-GUGCUACGucuCGCcaUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 35580 | 0.7 | 0.388436 |
Target: 5'- aGUC-GGCGCugGAaGCAGc-CGGUACg -3' miRNA: 3'- -UAGaCCGCGugCUaCGUCucGCCAUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 42491 | 0.66 | 0.602765 |
Target: 5'- uGUCUGGCGCugGcgcuguUGCGGAcccGCGaccaggauaccgcGUACg -3' miRNA: 3'- -UAGACCGCGugCu-----ACGUCU---CGC-------------CAUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 48036 | 0.69 | 0.415297 |
Target: 5'- gGUCUGGaugucucCGCuaugGAUGCAGAGCGG-ACc -3' miRNA: 3'- -UAGACC-------GCGug--CUACGUCUCGCCaUG- -5' |
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12348 | 5' | -56.6 | NC_003324.1 | + | 48325 | 0.71 | 0.320487 |
Target: 5'- --gUGuGUGCugGAUGUGGaAGCGGUACc -3' miRNA: 3'- uagAC-CGCGugCUACGUC-UCGCCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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