Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12358 | 5' | -52.9 | NC_003324.1 | + | 24039 | 0.67 | 0.747894 |
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Target: 5'- aUGCACuCGCCACCGUCuugaacgacuucGGCC-GCGu -3' miRNA: 3'- -AUGUG-GUGGUGGUAGuaa---------UCGGaCGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 5158 | 0.67 | 0.754296 |
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Target: 5'- cGCGCCGCUuaaGCUAUCA--GGCC-GCGu -3' miRNA: 3'- aUGUGGUGG---UGGUAGUaaUCGGaCGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 24845 | 0.67 | 0.764859 |
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Target: 5'- -uCACCACCGaCAUCGUaauCCUGCGc -3' miRNA: 3'- auGUGGUGGUgGUAGUAaucGGACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 1600 | 0.67 | 0.775278 |
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Target: 5'- cGCACCACUGCCGccggauccUCGcUGGCCcgGCc -3' miRNA: 3'- aUGUGGUGGUGGU--------AGUaAUCGGa-CGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 52000 | 0.66 | 0.788585 |
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Target: 5'- cACGCCGCCACCAUCucgaucucgucgauGuUCUGCu -3' miRNA: 3'- aUGUGGUGGUGGUAGuaau----------C-GGACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 45502 | 0.66 | 0.795632 |
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Target: 5'- gGCugCACCGCCgAUCAc-AGCCgucgGUGc -3' miRNA: 3'- aUGugGUGGUGG-UAGUaaUCGGa---CGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 11868 | 0.66 | 0.795632 |
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Target: 5'- gGCACCAUCACCAgugaaguaaagUCu---GuCCUGCGc -3' miRNA: 3'- aUGUGGUGGUGGU-----------AGuaauC-GGACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 49813 | 0.66 | 0.815261 |
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Target: 5'- gUGCGCcCGCUgaACCAUCcc--GCCUGCa -3' miRNA: 3'- -AUGUG-GUGG--UGGUAGuaauCGGACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 27115 | 1.07 | 0.002164 |
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Target: 5'- uUACACCACCACCAUCAUUAGCCUGCGc -3' miRNA: 3'- -AUGUGGUGGUGGUAGUAAUCGGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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