Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12358 | 5' | -52.9 | NC_003324.1 | + | 44010 | 0.68 | 0.67735 |
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Target: 5'- --gGCUGCCGCCGggaUCAUgccgAGCgCUGCGa -3' miRNA: 3'- augUGGUGGUGGU---AGUAa---UCG-GACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 45502 | 0.66 | 0.795632 |
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Target: 5'- gGCugCACCGCCgAUCAc-AGCCgucgGUGc -3' miRNA: 3'- aUGugGUGGUGG-UAGUaaUCGGa---CGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 47636 | 0.68 | 0.710845 |
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Target: 5'- gGCgACCggGCCGCCAUCGUcgAGCUgugGCa -3' miRNA: 3'- aUG-UGG--UGGUGGUAGUAa-UCGGa--CGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 48467 | 0.67 | 0.775278 |
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Target: 5'- cGCGCCAUCAaagcagaCAUCG--AGCgCUGCGa -3' miRNA: 3'- aUGUGGUGGUg------GUAGUaaUCG-GACGC- -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 49751 | 0.67 | 0.764859 |
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Target: 5'- gGCG-CACCACCAUCAcgUGGaacgCUGCa -3' miRNA: 3'- aUGUgGUGGUGGUAGUa-AUCg---GACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 49813 | 0.66 | 0.815261 |
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Target: 5'- gUGCGCcCGCUgaACCAUCcc--GCCUGCa -3' miRNA: 3'- -AUGUG-GUGG--UGGUAGuaauCGGACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 50530 | 0.67 | 0.775278 |
|
Target: 5'- cGCAgCCAgCACCcgCGUUGaCCUGCu -3' miRNA: 3'- aUGU-GGUgGUGGuaGUAAUcGGACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 52000 | 0.66 | 0.788585 |
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Target: 5'- cACGCCGCCACCAUCucgaucucgucgauGuUCUGCu -3' miRNA: 3'- aUGUGGUGGUGGUAGuaau----------C-GGACGc -5' |
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| 12358 | 5' | -52.9 | NC_003324.1 | + | 54271 | 0.67 | 0.742524 |
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Target: 5'- gGCAUCAgCGCCGagcCGUUgcuggaaGGCCUGCGu -3' miRNA: 3'- aUGUGGUgGUGGUa--GUAA-------UCGGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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