Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12367 | 5' | -53.8 | NC_003324.1 | + | 11585 | 0.66 | 0.807218 |
Target: 5'- cUCCCGcggcuccugccggagACugguGCGACGGCAGCg----- -3' miRNA: 3'- cAGGGU---------------UGu---CGCUGCCGUCGauaagc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 41120 | 0.66 | 0.803355 |
Target: 5'- gGUCCCucCuGgGAgCGGUAGCUGUUgCGu -3' miRNA: 3'- -CAGGGuuGuCgCU-GCCGUCGAUAA-GC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 30565 | 0.66 | 0.793569 |
Target: 5'- uUUCCGAgGGCcugcuCGGCGGCgccgAUUCGa -3' miRNA: 3'- cAGGGUUgUCGcu---GCCGUCGa---UAAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 13163 | 0.66 | 0.793569 |
Target: 5'- --gUCGGCGGCGGuCGGCGGCgg--CGg -3' miRNA: 3'- cagGGUUGUCGCU-GCCGUCGauaaGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 44573 | 0.66 | 0.783611 |
Target: 5'- -cCCCGuucgGCAGCGAcaCGGCGGCg----- -3' miRNA: 3'- caGGGU----UGUCGCU--GCCGUCGauaagc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 45449 | 0.66 | 0.783611 |
Target: 5'- uUUUCGACAcUGGCGGCAGgUAUUCc -3' miRNA: 3'- cAGGGUUGUcGCUGCCGUCgAUAAGc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 6143 | 0.66 | 0.773491 |
Target: 5'- aUCUCGAcCAGCGAUGGCAggauGCUGg--- -3' miRNA: 3'- cAGGGUU-GUCGCUGCCGU----CGAUaagc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 55023 | 0.66 | 0.763222 |
Target: 5'- --aCCAGguGCugGAUGaGCAGCUGUUCa -3' miRNA: 3'- cagGGUUguCG--CUGC-CGUCGAUAAGc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 15003 | 0.66 | 0.760113 |
Target: 5'- cUUCCAGCgcgagacgggcaaaGGCGACGGCAGg---UCGa -3' miRNA: 3'- cAGGGUUG--------------UCGCUGCCGUCgauaAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 30818 | 0.67 | 0.752814 |
Target: 5'- -gCCCAACGGuCGAUGGCGucggacGCU--UCGa -3' miRNA: 3'- caGGGUUGUC-GCUGCCGU------CGAuaAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 14597 | 0.67 | 0.752814 |
Target: 5'- --gCCGGCGGCGcagGCGGUAGCguggCGg -3' miRNA: 3'- cagGGUUGUCGC---UGCCGUCGauaaGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 1073 | 0.67 | 0.74228 |
Target: 5'- aGUCCUGGCGGCuGCGGUAccGCU--UCGa -3' miRNA: 3'- -CAGGGUUGUCGcUGCCGU--CGAuaAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 33979 | 0.67 | 0.74228 |
Target: 5'- -gCCCGACGGaaaGGCGG-AGCccgAUUCGg -3' miRNA: 3'- caGGGUUGUCg--CUGCCgUCGa--UAAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 29724 | 0.68 | 0.688134 |
Target: 5'- aUCCCGGCucaGGCGGCGGCguugCGc -3' miRNA: 3'- cAGGGUUGucgCUGCCGUCGauaaGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 49425 | 0.68 | 0.688134 |
Target: 5'- -gCCCGGCAGCGGCGcuuGGCUGaUCu -3' miRNA: 3'- caGGGUUGUCGCUGCcg-UCGAUaAGc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 35201 | 0.68 | 0.64375 |
Target: 5'- --gUCAACGGCGACGGC-GCUGgcgaCGa -3' miRNA: 3'- cagGGUUGUCGCUGCCGuCGAUaa--GC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 18474 | 0.69 | 0.6326 |
Target: 5'- -aCCCAAUcGUGACGGCuugcgAGCUcgUUGg -3' miRNA: 3'- caGGGUUGuCGCUGCCG-----UCGAuaAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 31270 | 0.69 | 0.610308 |
Target: 5'- uUUgCGGCGGCGACGGCuucUUGUUCGc -3' miRNA: 3'- cAGgGUUGUCGCUGCCGuc-GAUAAGC- -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 50780 | 0.69 | 0.610308 |
Target: 5'- uUCgUCGACGGCGACGGCAauGCUGc--- -3' miRNA: 3'- cAG-GGUUGUCGCUGCCGU--CGAUaagc -5' |
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12367 | 5' | -53.8 | NC_003324.1 | + | 29961 | 0.7 | 0.566031 |
Target: 5'- cGUCCUgcgUAGCGGCGGCAGCcggGUccgcaUCGa -3' miRNA: 3'- -CAGGGuu-GUCGCUGCCGUCGa--UA-----AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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