miRNA: 3'- cAGGGuUGUCG-CUGCCGUCGaUAAgC- -5'
miRNA: 3'- caggGUUGUCGCUGCCGUCGauaaGC- -5'
miRNA: 3'- -CAGGgU-------UGUCG-----------CUGCCGUCGAUAAGC- -5'
miRNA: 3'- -CAGgGUUGUCGcUGCCGUCGaua--AGC- -5'
miRNA: 3'- -CAGGGUUGUCGCUGCCGUCGAUAAGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12367 | 5' | -53.8 | NC_003324.1 | + | 8898 | 0.71 | 0.470304 |
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Target: 5'- uUCCCcACAGCgGGCGGCAGUgAUUgGu -3' miRNA: 3'- cAGGGuUGUCG-CUGCCGUCGaUAAgC- -5' |
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| 12367 | 5' | -53.8 | NC_003324.1 | + | 35029 | 0.72 | 0.450128 |
|
Target: 5'- ---gCAGCGGCGGCGGCAGCag--CGg -3' miRNA: 3'- caggGUUGUCGCUGCCGUCGauaaGC- -5' |
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| 12367 | 5' | -53.8 | NC_003324.1 | + | 29212 | 0.74 | 0.356177 |
|
Target: 5'- cGUCCgGcagcacuACGGUgcgcucaccaaGACGGCGGCUGUUCGg -3' miRNA: 3'- -CAGGgU-------UGUCG-----------CUGCCGUCGAUAAGC- -5' |
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| 12367 | 5' | -53.8 | NC_003324.1 | + | 30612 | 0.74 | 0.323777 |
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Target: 5'- cGUCgCAGCGGCaGCGGCAGCgaaggUCGc -3' miRNA: 3'- -CAGgGUUGUCGcUGCCGUCGaua--AGC- -5' |
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| 12367 | 5' | -53.8 | NC_003324.1 | + | 31552 | 1.1 | 0.001232 |
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Target: 5'- cGUCCCAACAGCGACGGCAGCUAUUCGa -3' miRNA: 3'- -CAGGGUUGUCGCUGCCGUCGAUAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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