Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12368 | 5' | -54.6 | NC_003324.1 | + | 51130 | 0.66 | 0.786709 |
Target: 5'- uCUGCCucGUGgagUUCcaaCCCGAacucuucgucUUGGGCCa -3' miRNA: 3'- -GAUGGu-CGCa--AAGc--GGGCU----------AACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 50586 | 0.67 | 0.714485 |
Target: 5'- gUGCCAGCGcugUUUCaaaaGCUgGGUgaucugguugUGGGCCa -3' miRNA: 3'- gAUGGUCGC---AAAG----CGGgCUA----------ACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 49108 | 0.7 | 0.508575 |
Target: 5'- gCUGCCGGUGacgcgaUCGCCaaGAUggcGGGCCg -3' miRNA: 3'- -GAUGGUCGCaa----AGCGGg-CUAa--CCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 48617 | 0.75 | 0.300442 |
Target: 5'- uCU-CCuucuGgGUUUCGCCCgGAUUGGGCUu -3' miRNA: 3'- -GAuGGu---CgCAAAGCGGG-CUAACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 48260 | 0.7 | 0.529613 |
Target: 5'- -aACCAGCGaacgCGgCCGAUaGGGaCCa -3' miRNA: 3'- gaUGGUCGCaaa-GCgGGCUAaCCC-GG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 40964 | 0.69 | 0.594367 |
Target: 5'- --uCCAGCG-UUCGUugaagUCGAUcGGGCCg -3' miRNA: 3'- gauGGUCGCaAAGCG-----GGCUAaCCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 40571 | 0.67 | 0.725137 |
Target: 5'- aCUACCGuGuCGUcaugUCGCCCGAUgugguUGuGGUCc -3' miRNA: 3'- -GAUGGU-C-GCAa---AGCGGGCUA-----AC-CCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 34495 | 0.66 | 0.766707 |
Target: 5'- -gGCCGGCGUcUUGUUCGuc-GGGUCc -3' miRNA: 3'- gaUGGUCGCAaAGCGGGCuaaCCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 32344 | 0.66 | 0.786709 |
Target: 5'- cCUGCCuAGCGgcaUCGCCCGGcuuccUGuaGCCg -3' miRNA: 3'- -GAUGG-UCGCaa-AGCGGGCUa----ACc-CGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 32299 | 1.12 | 0.000829 |
Target: 5'- gCUACCAGCGUUUCGCCCGAUUGGGCCu -3' miRNA: 3'- -GAUGGUCGCAAAGCGGGCUAACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 31679 | 0.68 | 0.660233 |
Target: 5'- -cGCCAGcCGUUauggucugUCuuuCCCGA-UGGGCCa -3' miRNA: 3'- gaUGGUC-GCAA--------AGc--GGGCUaACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 30986 | 0.74 | 0.315668 |
Target: 5'- --uCCGGCGUg--GUCgGAUUGGGCCa -3' miRNA: 3'- gauGGUCGCAaagCGGgCUAACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 30400 | 0.66 | 0.746152 |
Target: 5'- -gACCAGgauaCGgacggCuUCCGAUUGGGCCa -3' miRNA: 3'- gaUGGUC----GCaaa--GcGGGCUAACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 30039 | 0.69 | 0.60203 |
Target: 5'- gCUGCCGccgcuacgcaggacGCcccUCGCCCGuccggaccGUUGGGCCg -3' miRNA: 3'- -GAUGGU--------------CGcaaAGCGGGC--------UAACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 28168 | 0.68 | 0.647065 |
Target: 5'- uUGCCGGCuuccuucugUGCCUGAgUGGGCUc -3' miRNA: 3'- gAUGGUCGcaaa-----GCGGGCUaACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 17246 | 0.69 | 0.572568 |
Target: 5'- -cGCCAGCGccUCGUCaaucgcUGGGCCa -3' miRNA: 3'- gaUGGUCGCaaAGCGGgcua--ACCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 14727 | 0.73 | 0.347844 |
Target: 5'- gUACUGGCuGUUggUCGCCUGAUUgccggaaaGGGCCg -3' miRNA: 3'- gAUGGUCG-CAA--AGCGGGCUAA--------CCCGG- -5' |
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12368 | 5' | -54.6 | NC_003324.1 | + | 12815 | 0.68 | 0.671179 |
Target: 5'- --uCCAGCGUUUCGacgcgCCGcaagGGGCUc -3' miRNA: 3'- gauGGUCGCAAAGCg----GGCuaa-CCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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