miRNA: 3'- -GCGG------CUCCGa---------CGGcCGCUUCCGCUGc -5'
miRNA: 3'- gCGGcucCGACGGC-CGCUUCCgCUGC- -5'
miRNA: 3'- gC-GGCUCCGACGGCCGcuucCGCugc -5'
miRNA: 3'- gCGGC----------UCCGACGGCCGcu----UCCGCuGC- -5'
miRNA: 3'- gC-GGCUCCGaC-GGCCGc-UUCCGCUgc -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12369 | 5' | -62.4 | NC_003324.1 | + | 49114 | 0.67 | 0.348641 |
|
Target: 5'- cCGCCgcgggaGAGGUgagaagcaugGCC-GCGAGGGCGGCc -3' miRNA: 3'- -GCGG------CUCCGa---------CGGcCGCUUCCGCUGc -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 26292 | 0.67 | 0.340795 |
|
Target: 5'- uGCU---GCUGCCGaGCGAGGG-GACGa -3' miRNA: 3'- gCGGcucCGACGGC-CGCUUCCgCUGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 20251 | 0.67 | 0.340795 |
|
Target: 5'- uGaCCGAGGCUGCCGGCa---GCGu-- -3' miRNA: 3'- gC-GGCUCCGACGGCCGcuucCGCugc -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 20305 | 0.67 | 0.333077 |
|
Target: 5'- gGCUGcauuccaucuGGGCUGCCGGCcauaccGGGCG-CGu -3' miRNA: 3'- gCGGC----------UCCGACGGCCGcu----UCCGCuGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 32220 | 0.66 | 0.398372 |
|
Target: 5'- aGcCCGGGGCgGcCCGGCccGAGGCGGa- -3' miRNA: 3'- gC-GGCUCCGaC-GGCCGc-UUCCGCUgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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