Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12375 | 5' | -53 | NC_003324.1 | + | 48906 | 0.72 | 0.50556 |
Target: 5'- aCUCCGCCACCcgccacaagaucGUCGaccuggUCAAGGcCGUCg -3' miRNA: 3'- gGAGGCGGUGG------------CAGC------AGUUCUuGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 8282 | 0.66 | 0.874625 |
Target: 5'- uCCguagUCGUCGCCGUCG-CAGccAGCGUCa -3' miRNA: 3'- -GGa---GGCGGUGGCAGCaGUUc-UUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 44140 | 0.66 | 0.869889 |
Target: 5'- cCCUuuGCCucGCCGuacacauccuugaugUCGUCGGcGAugGUCu -3' miRNA: 3'- -GGAggCGG--UGGC---------------AGCAGUU-CUugUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 34407 | 0.66 | 0.866682 |
Target: 5'- aCU-CGCCGCCGUCcUCG---GCAUCg -3' miRNA: 3'- gGAgGCGGUGGCAGcAGUucuUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 6904 | 0.66 | 0.841405 |
Target: 5'- cUCUUCGCCACCGcggUCGguaUCAAGGuucaGUCc -3' miRNA: 3'- -GGAGGCGGUGGC---AGC---AGUUCUug--UAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 35106 | 0.67 | 0.823433 |
Target: 5'- -aUCUGCCuuuCCGUCGUCGccAGcgcCGUCg -3' miRNA: 3'- ggAGGCGGu--GGCAGCAGU--UCuu-GUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 12883 | 0.67 | 0.823433 |
Target: 5'- aCUUCGCCAUCGUCGaUggGcGCGg- -3' miRNA: 3'- gGAGGCGGUGGCAGCaGuuCuUGUag -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 35572 | 0.67 | 0.814138 |
Target: 5'- gCCUgCGCCugCGUCuUCu-GcGCAUCg -3' miRNA: 3'- -GGAgGCGGugGCAGcAGuuCuUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 39422 | 0.67 | 0.794981 |
Target: 5'- aCUgUGUCACCGUucuUGUCGAGGAUGUa -3' miRNA: 3'- gGAgGCGGUGGCA---GCAGUUCUUGUAg -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 50382 | 0.68 | 0.77514 |
Target: 5'- --gCCGUCGCCGUUGUCccGGGCcgCg -3' miRNA: 3'- ggaGGCGGUGGCAGCAGuuCUUGuaG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 56023 | 0.68 | 0.764989 |
Target: 5'- ----aGCgCGCCGUgGUCAAGAAgAUCg -3' miRNA: 3'- ggaggCG-GUGGCAgCAGUUCUUgUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 19909 | 0.69 | 0.712407 |
Target: 5'- -aUUCGCCGCCGU-GUCAAucACAUUa -3' miRNA: 3'- ggAGGCGGUGGCAgCAGUUcuUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 34823 | 0.69 | 0.701606 |
Target: 5'- uCCUUCGCCugcagcucGCCGUCGUCGAacuGC-UCu -3' miRNA: 3'- -GGAGGCGG--------UGGCAGCAGUUcu-UGuAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 21815 | 0.69 | 0.67981 |
Target: 5'- uUUCuCGUCGCCGUCGUCcgccuucuGCAUCa -3' miRNA: 3'- gGAG-GCGGUGGCAGCAGuucu----UGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 1194 | 0.7 | 0.65783 |
Target: 5'- -aUCCgGCCGCgauccgaaCGUCGUCAAGAAaAUCg -3' miRNA: 3'- ggAGG-CGGUG--------GCAGCAGUUCUUgUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 40166 | 0.71 | 0.602641 |
Target: 5'- -gUCCGaCACCuUCGaCAGGAACAUCa -3' miRNA: 3'- ggAGGCgGUGGcAGCaGUUCUUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 35160 | 0.72 | 0.50556 |
Target: 5'- aCCUUCGgCGCCGUCGUCuuccAGucccuCGUCa -3' miRNA: 3'- -GGAGGCgGUGGCAGCAGu---UCuu---GUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 1478 | 0.67 | 0.794981 |
Target: 5'- uUUCCGCCAUCuUCGUCuauGGCAUUg -3' miRNA: 3'- gGAGGCGGUGGcAGCAGuucUUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 34820 | 1.13 | 0.001072 |
Target: 5'- aCCUCCGCCACCGUCGUCAAGAACAUCg -3' miRNA: 3'- -GGAGGCGGUGGCAGCAGUUCUUGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 2613 | 0.7 | 0.613666 |
Target: 5'- gCCgUCCGUCGCCuguUCGUCGGuGGCGUCa -3' miRNA: 3'- -GG-AGGCGGUGGc--AGCAGUUcUUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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