Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12375 | 5' | -53 | NC_003324.1 | + | 10090 | 0.69 | 0.690736 |
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Target: 5'- uUUCgGCCGCCGUCGUUAuc--CGUCc -3' miRNA: 3'- gGAGgCGGUGGCAGCAGUucuuGUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 25368 | 0.69 | 0.723129 |
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Target: 5'- aCCUCCGCaga-GaUCGUCAaggcugcauGGGACAUCg -3' miRNA: 3'- -GGAGGCGguggC-AGCAGU---------UCUUGUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 44383 | 0.68 | 0.744288 |
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Target: 5'- gCCg-CGCCACCGcgcccgguUCGUCGAGAcAgAUCg -3' miRNA: 3'- -GGagGCGGUGGC--------AGCAGUUCU-UgUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 34055 | 0.68 | 0.744288 |
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Target: 5'- gCUCCGCCuuuCCGUCGggCGGGuGCu-- -3' miRNA: 3'- gGAGGCGGu--GGCAGCa-GUUCuUGuag -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 24076 | 0.67 | 0.785141 |
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Target: 5'- cCCUaCCGCCGCCcuaccagcgCGUCGAGcGCGa- -3' miRNA: 3'- -GGA-GGCGGUGGca-------GCAGUUCuUGUag -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 52195 | 0.67 | 0.823433 |
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Target: 5'- gCCUUCGCCgACC-UCGUCAAaauaauCGUCg -3' miRNA: 3'- -GGAGGCGG-UGGcAGCAGUUcuu---GUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 34820 | 1.13 | 0.001072 |
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Target: 5'- aCCUCCGCCACCGUCGUCAAGAACAUCg -3' miRNA: 3'- -GGAGGCGGUGGCAGCAGUUCUUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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