Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12375 | 5' | -53 | NC_003324.1 | + | 44140 | 0.66 | 0.869889 |
Target: 5'- cCCUuuGCCucGCCGuacacauccuugaugUCGUCGGcGAugGUCu -3' miRNA: 3'- -GGAggCGG--UGGC---------------AGCAGUU-CUugUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 44383 | 0.68 | 0.744288 |
Target: 5'- gCCg-CGCCACCGcgcccgguUCGUCGAGAcAgAUCg -3' miRNA: 3'- -GGagGCGGUGGC--------AGCAGUUCU-UgUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 48906 | 0.72 | 0.50556 |
Target: 5'- aCUCCGCCACCcgccacaagaucGUCGaccuggUCAAGGcCGUCg -3' miRNA: 3'- gGAGGCGGUGG------------CAGC------AGUUCUuGUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 50382 | 0.68 | 0.77514 |
Target: 5'- --gCCGUCGCCGUUGUCccGGGCcgCg -3' miRNA: 3'- ggaGGCGGUGGCAGCAGuuCUUGuaG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 52195 | 0.67 | 0.823433 |
Target: 5'- gCCUUCGCCgACC-UCGUCAAaauaauCGUCg -3' miRNA: 3'- -GGAGGCGG-UGGcAGCAGUUcuu---GUAG- -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 55838 | 0.7 | 0.673231 |
Target: 5'- uUUCCGCCACCGaUCGuauccggauccuguaUCcGAGAACGUg -3' miRNA: 3'- gGAGGCGGUGGC-AGC---------------AG-UUCUUGUAg -5' |
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12375 | 5' | -53 | NC_003324.1 | + | 56023 | 0.68 | 0.764989 |
Target: 5'- ----aGCgCGCCGUgGUCAAGAAgAUCg -3' miRNA: 3'- ggaggCG-GUGGCAgCAGUUCUUgUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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