Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12375 | 5' | -53 | NC_003324.1 | + | 52195 | 0.67 | 0.823433 |
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Target: 5'- gCCUUCGCCgACC-UCGUCAAaauaauCGUCg -3' miRNA: 3'- -GGAGGCGG-UGGcAGCAGUUcuu---GUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 35106 | 0.67 | 0.823433 |
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Target: 5'- -aUCUGCCuuuCCGUCGUCGccAGcgcCGUCg -3' miRNA: 3'- ggAGGCGGu--GGCAGCAGU--UCuu-GUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 12883 | 0.67 | 0.823433 |
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Target: 5'- aCUUCGCCAUCGUCGaUggGcGCGg- -3' miRNA: 3'- gGAGGCGGUGGCAGCaGuuCuUGUag -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 6904 | 0.66 | 0.841405 |
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Target: 5'- cUCUUCGCCACCGcggUCGguaUCAAGGuucaGUCc -3' miRNA: 3'- -GGAGGCGGUGGC---AGC---AGUUCUug--UAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 34407 | 0.66 | 0.866682 |
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Target: 5'- aCU-CGCCGCCGUCcUCG---GCAUCg -3' miRNA: 3'- gGAgGCGGUGGCAGcAGUucuUGUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 44140 | 0.66 | 0.869889 |
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Target: 5'- cCCUuuGCCucGCCGuacacauccuugaugUCGUCGGcGAugGUCu -3' miRNA: 3'- -GGAggCGG--UGGC---------------AGCAGUU-CUugUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 8282 | 0.66 | 0.874625 |
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Target: 5'- uCCguagUCGUCGCCGUCG-CAGccAGCGUCa -3' miRNA: 3'- -GGa---GGCGGUGGCAGCaGUUc-UUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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