miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12375 5' -53 NC_003324.1 + 55838 0.7 0.673231
Target:  5'- uUUCCGCCACCGaUCGuauccggauccuguaUCcGAGAACGUg -3'
miRNA:   3'- gGAGGCGGUGGC-AGC---------------AG-UUCUUGUAg -5'
12375 5' -53 NC_003324.1 + 1194 0.7 0.65783
Target:  5'- -aUCCgGCCGCgauccgaaCGUCGUCAAGAAaAUCg -3'
miRNA:   3'- ggAGG-CGGUG--------GCAGCAGUUCUUgUAG- -5'
12375 5' -53 NC_003324.1 + 2613 0.7 0.613666
Target:  5'- gCCgUCCGUCGCCuguUCGUCGGuGGCGUCa -3'
miRNA:   3'- -GG-AGGCGGUGGc--AGCAGUUcUUGUAG- -5'
12375 5' -53 NC_003324.1 + 40166 0.71 0.602641
Target:  5'- -gUCCGaCACCuUCGaCAGGAACAUCa -3'
miRNA:   3'- ggAGGCgGUGGcAGCaGUUCUUGUAG- -5'
12375 5' -53 NC_003324.1 + 48906 0.72 0.50556
Target:  5'- aCUCCGCCACCcgccacaagaucGUCGaccuggUCAAGGcCGUCg -3'
miRNA:   3'- gGAGGCGGUGG------------CAGC------AGUUCUuGUAG- -5'
12375 5' -53 NC_003324.1 + 35160 0.72 0.50556
Target:  5'- aCCUUCGgCGCCGUCGUCuuccAGucccuCGUCa -3'
miRNA:   3'- -GGAGGCgGUGGCAGCAGu---UCuu---GUAG- -5'
12375 5' -53 NC_003324.1 + 34820 1.13 0.001072
Target:  5'- aCCUCCGCCACCGUCGUCAAGAACAUCg -3'
miRNA:   3'- -GGAGGCGGUGGCAGCAGUUCUUGUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.