Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12375 | 5' | -53 | NC_003324.1 | + | 56023 | 0.68 | 0.764989 |
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Target: 5'- ----aGCgCGCCGUgGUCAAGAAgAUCg -3' miRNA: 3'- ggaggCG-GUGGCAgCAGUUCUUgUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 19909 | 0.69 | 0.712407 |
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Target: 5'- -aUUCGCCGCCGU-GUCAAucACAUUa -3' miRNA: 3'- ggAGGCGGUGGCAgCAGUUcuUGUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 34823 | 0.69 | 0.701606 |
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Target: 5'- uCCUUCGCCugcagcucGCCGUCGUCGAacuGC-UCu -3' miRNA: 3'- -GGAGGCGG--------UGGCAGCAGUUcu-UGuAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 21815 | 0.69 | 0.67981 |
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Target: 5'- uUUCuCGUCGCCGUCGUCcgccuucuGCAUCa -3' miRNA: 3'- gGAG-GCGGUGGCAGCAGuucu----UGUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 1194 | 0.7 | 0.65783 |
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Target: 5'- -aUCCgGCCGCgauccgaaCGUCGUCAAGAAaAUCg -3' miRNA: 3'- ggAGG-CGGUG--------GCAGCAGUUCUUgUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 40166 | 0.71 | 0.602641 |
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Target: 5'- -gUCCGaCACCuUCGaCAGGAACAUCa -3' miRNA: 3'- ggAGGCgGUGGcAGCaGUUCUUGUAG- -5' |
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| 12375 | 5' | -53 | NC_003324.1 | + | 48906 | 0.72 | 0.50556 |
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Target: 5'- aCUCCGCCACCcgccacaagaucGUCGaccuggUCAAGGcCGUCg -3' miRNA: 3'- gGAGGCGGUGG------------CAGC------AGUUCUuGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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