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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12376 | 3' | -57.9 | NC_003324.1 | + | 32536 | 0.72 | 0.270878 |
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Target: 5'- aGGCGACGAgGG-CAuGGCUGCcGUUGCc -3' miRNA: 3'- -UCGCUGCUgCUaGU-CCGGCGaCGACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 26319 | 0.73 | 0.232685 |
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Target: 5'- cGUGGCaGAUGGUCAGGgagaccuacUUGCUGCUGCc -3' miRNA: 3'- uCGCUG-CUGCUAGUCC---------GGCGACGACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 5098 | 0.73 | 0.244886 |
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Target: 5'- cGCGAuCGACGAUCuGcGCCGCUuuuucCUGCc -3' miRNA: 3'- uCGCU-GCUGCUAGuC-CGGCGAc----GACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 35483 | 0.73 | 0.23872 |
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Target: 5'- uGGgGACGGCGAUC--GCCGUaccgGCUGCu -3' miRNA: 3'- -UCgCUGCUGCUAGucCGGCGa---CGACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 13164 | 0.73 | 0.215346 |
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Target: 5'- cGuCGGCGGCGGUCGgcGGCgGCgguaccgGCUGCa -3' miRNA: 3'- uC-GCUGCUGCUAGU--CCGgCGa------CGACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 29460 | 0.75 | 0.174458 |
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Target: 5'- -uCGACGAUGA-CAcGGCCGCcugGCUGCg -3' miRNA: 3'- ucGCUGCUGCUaGU-CCGGCGa--CGACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 24001 | 0.77 | 0.126069 |
|
Target: 5'- uGGUagGGCGGCGGUaGGGCUGCUGCgUGCg -3' miRNA: 3'- -UCG--CUGCUGCUAgUCCGGCGACG-ACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 32911 | 0.8 | 0.080766 |
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Target: 5'- cAGUGGCGAUGAUCgAGGCgGCUGCccgccaUGCg -3' miRNA: 3'- -UCGCUGCUGCUAG-UCCGgCGACG------ACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 25593 | 0.82 | 0.057476 |
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Target: 5'- cGGUGAUGACauaGUCugucGGCCGCUGCUGCg -3' miRNA: 3'- -UCGCUGCUGc--UAGu---CCGGCGACGACG- -5' |
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| 12376 | 3' | -57.9 | NC_003324.1 | + | 35124 | 1.1 | 0.000464 |
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Target: 5'- cAGCGACGACGAUCAGGCCGCUGCUGCc -3' miRNA: 3'- -UCGCUGCUGCUAGUCCGGCGACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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