Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12379 | 3' | -56.7 | NC_003324.1 | + | 47480 | 0.66 | 0.717899 |
Target: 5'- uCCGCCU-GGCaGGCCUgcaucgcaUUUGUCGAaCCa -3' miRNA: 3'- -GGUGGAgCCG-CUGGA--------GGACAGCUaGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 7975 | 0.66 | 0.717899 |
Target: 5'- aUCAUucaUUCGGCGACCUugaCCUGaCGGUgaCCu -3' miRNA: 3'- -GGUG---GAGCCGCUGGA---GGACaGCUA--GG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 48395 | 0.66 | 0.711675 |
Target: 5'- aCCcCCUCGcGCaGCUUCUugcgaaugccgcgcuUGUCGAUCUg -3' miRNA: 3'- -GGuGGAGC-CGcUGGAGG---------------ACAGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 29963 | 0.66 | 0.697052 |
Target: 5'- uCCugCguagCGGCGGCagccgggUCCgcaUCGAUCCc -3' miRNA: 3'- -GGugGa---GCCGCUGg------AGGac-AGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 48883 | 0.66 | 0.686535 |
Target: 5'- -gGCCUCGGUGGCaccuuccggCUUGUCGAa-- -3' miRNA: 3'- ggUGGAGCCGCUGga-------GGACAGCUagg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 46131 | 0.66 | 0.686535 |
Target: 5'- cUCAUCUCGGCaaguGGCCUUCga-CGAUCa -3' miRNA: 3'- -GGUGGAGCCG----CUGGAGGacaGCUAGg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 37557 | 0.66 | 0.686535 |
Target: 5'- aCUGCCUCuGUGACCUugcCCgugagggcGUCGAUCa -3' miRNA: 3'- -GGUGGAGcCGCUGGA---GGa-------CAGCUAGg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 23422 | 0.66 | 0.686535 |
Target: 5'- gCCGCgUCGGUGGCgC-CCUgGUUGA-CCa -3' miRNA: 3'- -GGUGgAGCCGCUG-GaGGA-CAGCUaGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 56211 | 0.66 | 0.67491 |
Target: 5'- cUCGCCUaucgcucgaucggCGGCGACaUCCUGcaUCcGUCCg -3' miRNA: 3'- -GGUGGA-------------GCCGCUGgAGGAC--AGcUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 25490 | 0.67 | 0.665364 |
Target: 5'- gUCGCCg-GGCGcuUCUCCUuGUCGAguuUCCg -3' miRNA: 3'- -GGUGGagCCGCu-GGAGGA-CAGCU---AGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 43875 | 0.67 | 0.65473 |
Target: 5'- gCGCCUugcCGGCGuucCCggcacCCUGcUCGAUCUg -3' miRNA: 3'- gGUGGA---GCCGCu--GGa----GGAC-AGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 50703 | 0.67 | 0.644076 |
Target: 5'- cCCGCCUCGGCGGuaaCUCuaugCUGUgCGAa-- -3' miRNA: 3'- -GGUGGAGCCGCUg--GAG----GACA-GCUagg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 35665 | 0.67 | 0.644076 |
Target: 5'- uCCuuCUUGGCGGCUUCCUcgggGUuuucuucggccUGAUCCg -3' miRNA: 3'- -GGugGAGCCGCUGGAGGA----CA-----------GCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 17402 | 0.67 | 0.643009 |
Target: 5'- gUCACCUCgaucgguGGUGACCaggUCCUGcC-AUCCg -3' miRNA: 3'- -GGUGGAG-------CCGCUGG---AGGACaGcUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 17537 | 0.67 | 0.633411 |
Target: 5'- cCCGCgUCGGCGAUCggaaCCaaa-GGUCCg -3' miRNA: 3'- -GGUGgAGCCGCUGGa---GGacagCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 23794 | 0.67 | 0.633411 |
Target: 5'- uCgGCCUCGGCGAgcuuCUUCUUGaCGuugCCg -3' miRNA: 3'- -GgUGGAGCCGCU----GGAGGACaGCua-GG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 48166 | 0.67 | 0.633411 |
Target: 5'- gCCACgUCGGCGcgauCCUCaUGaagCGAUCg -3' miRNA: 3'- -GGUGgAGCCGCu---GGAGgACa--GCUAGg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 19109 | 0.67 | 0.622746 |
Target: 5'- uCCuCUUCGGC-AUUgcgCUUGUCGAUCCc -3' miRNA: 3'- -GGuGGAGCCGcUGGa--GGACAGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 28624 | 0.67 | 0.622746 |
Target: 5'- gCCGCCauguugagcgCGGCaGGCCUCagUGgucUCGGUCCg -3' miRNA: 3'- -GGUGGa---------GCCG-CUGGAGg-AC---AGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 5327 | 0.67 | 0.612089 |
Target: 5'- gCCGCUggugCGGCGGCCaaCUGaugcUCGAaCCg -3' miRNA: 3'- -GGUGGa---GCCGCUGGagGAC----AGCUaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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