Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12379 | 3' | -56.7 | NC_003324.1 | + | 2995 | 0.68 | 0.601448 |
Target: 5'- gUCGCUUCGaGCGgcccaguaauucGCCUCUaugcgGUCGAUCUg -3' miRNA: 3'- -GGUGGAGC-CGC------------UGGAGGa----CAGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 32489 | 0.68 | 0.559208 |
Target: 5'- -aGCCUCGGCGAUCacggUGUCGAcgaugucgcUCCa -3' miRNA: 3'- ggUGGAGCCGCUGGagg-ACAGCU---------AGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 27971 | 0.68 | 0.548767 |
Target: 5'- gCACCUCGGUaccgGGCUUCacgaagaUGUCGAuauggUCCu -3' miRNA: 3'- gGUGGAGCCG----CUGGAGg------ACAGCU-----AGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 16241 | 0.68 | 0.548767 |
Target: 5'- cCCGCCUCGGUG-CCagcgaugCCUGgcgCGGcCCc -3' miRNA: 3'- -GGUGGAGCCGCuGGa------GGACa--GCUaGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 38421 | 0.69 | 0.528077 |
Target: 5'- gCCGCCcCGGUGcCCUCgUGaUCGAgacgCCc -3' miRNA: 3'- -GGUGGaGCCGCuGGAGgAC-AGCUa---GG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 38561 | 0.69 | 0.51784 |
Target: 5'- uUCACCaggCGGuCGGCCUC--GUCGAUCa -3' miRNA: 3'- -GGUGGa--GCC-GCUGGAGgaCAGCUAGg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 21861 | 0.69 | 0.507683 |
Target: 5'- gCCuCCUCGGCGGCCUuggCCUucuucgggGUCGAc-- -3' miRNA: 3'- -GGuGGAGCCGCUGGA---GGA--------CAGCUagg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 30556 | 0.69 | 0.497612 |
Target: 5'- gCCugCUCGGCGGCg-CCgauUCGAcCCg -3' miRNA: 3'- -GGugGAGCCGCUGgaGGac-AGCUaGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 34635 | 0.71 | 0.429895 |
Target: 5'- gCACCUCGGCGuCCagCaUGUCGAa-- -3' miRNA: 3'- gGUGGAGCCGCuGGagG-ACAGCUagg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 15665 | 0.71 | 0.429895 |
Target: 5'- cCCGCCUUGGC-AUCUgCCgUGUCGGcCCa -3' miRNA: 3'- -GGUGGAGCCGcUGGA-GG-ACAGCUaGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 37721 | 1.13 | 0.000534 |
Target: 5'- aCCACCUCGGCGACCUCCUGUCGAUCCc -3' miRNA: 3'- -GGUGGAGCCGCUGGAGGACAGCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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