Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12388 | 3' | -56.2 | NC_003324.1 | + | 26876 | 0.68 | 0.545122 |
Target: 5'- -cCAAGCGCaCGGCCgcGUCuGCcaaGCCGAa -3' miRNA: 3'- aaGUUUGCG-GCCGG--UAGuCGc--UGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 27956 | 0.68 | 0.534427 |
Target: 5'- -gUAAugGCCGGCUAcgcCAGCGAggaCGAa -3' miRNA: 3'- aaGUUugCGGCCGGUa--GUCGCUg--GCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 38735 | 0.68 | 0.534427 |
Target: 5'- -cCAGGCGCgcuUGGCCucugaaAUCgGGCGGCCGAu -3' miRNA: 3'- aaGUUUGCG---GCCGG------UAG-UCGCUGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 17195 | 0.68 | 0.513268 |
Target: 5'- gUCGGACG-CGGCUuUCGGCGuucuugagcGCCGAg -3' miRNA: 3'- aAGUUUGCgGCCGGuAGUCGC---------UGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 47945 | 0.68 | 0.513268 |
Target: 5'- gUCcuGACGCCGGUCAgguacgguuUCAGCGGCa-- -3' miRNA: 3'- aAGu-UUGCGGCCGGU---------AGUCGCUGgcu -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 23838 | 0.68 | 0.509077 |
Target: 5'- gUCGGACGuuucguaguuuCCGGCCGgcgucuuguagagCAGCGgACCGAg -3' miRNA: 3'- aAGUUUGC-----------GGCCGGUa------------GUCGC-UGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 31105 | 0.68 | 0.502818 |
Target: 5'- -cCGAccGCGCCGGUCGcuaccaAGCGAUCGAc -3' miRNA: 3'- aaGUU--UGCGGCCGGUag----UCGCUGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 29538 | 0.69 | 0.486295 |
Target: 5'- -cCAGGCgGCCGuGUCAUCgucgagggggagaauGGCGACCGGu -3' miRNA: 3'- aaGUUUG-CGGC-CGGUAG---------------UCGCUGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 52377 | 0.69 | 0.481184 |
Target: 5'- -gCAGACGCCGGCCGccccgaaUCGgGCGGuaGAa -3' miRNA: 3'- aaGUUUGCGGCCGGU-------AGU-CGCUggCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 21945 | 0.69 | 0.472051 |
Target: 5'- --uGGAUGCgGGCCucucGUCGGCGGCgCGAu -3' miRNA: 3'- aagUUUGCGgCCGG----UAGUCGCUG-GCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 52273 | 0.69 | 0.462008 |
Target: 5'- aUCGGGCgguGCCGGUCAUCAGCuuGCCu- -3' miRNA: 3'- aAGUUUG---CGGCCGGUAGUCGc-UGGcu -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 4472 | 0.69 | 0.462008 |
Target: 5'- aUUCAGGCGCCGGaugcggcucaCAUCcucuacggcGGCGACgCGAc -3' miRNA: 3'- -AAGUUUGCGGCCg---------GUAG---------UCGCUG-GCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 55376 | 0.69 | 0.442266 |
Target: 5'- aUUCGAGCGCCuuauccggaaGCCAaaauUCAGCG-CCGAa -3' miRNA: 3'- -AAGUUUGCGGc---------CGGU----AGUCGCuGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 2510 | 0.69 | 0.442266 |
Target: 5'- -aCAAGcCGCCGG-CGUCGGCGGCUu- -3' miRNA: 3'- aaGUUU-GCGGCCgGUAGUCGCUGGcu -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 8871 | 0.7 | 0.413572 |
Target: 5'- -gCGAACuGUaCGGCCAUCAGCGGCa-- -3' miRNA: 3'- aaGUUUG-CG-GCCGGUAGUCGCUGgcu -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 18982 | 0.7 | 0.404265 |
Target: 5'- gUUCGAGCGCgCaGCCGaacGCGACCGAg -3' miRNA: 3'- -AAGUUUGCG-GcCGGUaguCGCUGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 49205 | 0.7 | 0.404265 |
Target: 5'- aUCGAGCgGCCcGCCAUCuuGGCGAUCGc -3' miRNA: 3'- aAGUUUG-CGGcCGGUAG--UCGCUGGCu -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 6600 | 0.71 | 0.368392 |
Target: 5'- -gCAGAC-CCuGCCGuggcUCAGCGGCCGAg -3' miRNA: 3'- aaGUUUGcGGcCGGU----AGUCGCUGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 18092 | 0.71 | 0.351293 |
Target: 5'- ---uGGCGCCGGCCaAUCgguauccgaAGCGAUCGAc -3' miRNA: 3'- aaguUUGCGGCCGG-UAG---------UCGCUGGCU- -5' |
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12388 | 3' | -56.2 | NC_003324.1 | + | 47985 | 0.72 | 0.311054 |
Target: 5'- -gCGGAUaaGCCGGCCAUCGGCGcaGCaCGGu -3' miRNA: 3'- aaGUUUG--CGGCCGGUAGUCGC--UG-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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