Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12389 | 5' | -54.9 | NC_003324.1 | + | 54491 | 0.72 | 0.402114 |
Target: 5'- gCC-CGGCAGCgGUCC-CGggGAGUUGCCg -3' miRNA: 3'- -GGcGUCGUCG-UAGGaGCa-CUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 54316 | 0.68 | 0.629217 |
Target: 5'- aCCGguGCcGCGcCUUCGcuuUGAGCaGCCg -3' miRNA: 3'- -GGCguCGuCGUaGGAGC---ACUCGaUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 53654 | 0.71 | 0.439884 |
Target: 5'- aCCGCcguGCGGCA-CCUUG-GAGC-GCCg -3' miRNA: 3'- -GGCGu--CGUCGUaGGAGCaCUCGaUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 53293 | 0.66 | 0.737471 |
Target: 5'- uCCGCAGCuu--UCgUUGUGAagccggccgccaGCUACCa -3' miRNA: 3'- -GGCGUCGucguAGgAGCACU------------CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 47018 | 0.72 | 0.402114 |
Target: 5'- aCCGCAGCGGCuaUCUCGUcGAuugaggcgGUUGCCu -3' miRNA: 3'- -GGCGUCGUCGuaGGAGCA-CU--------CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 45981 | 0.67 | 0.705593 |
Target: 5'- -aGCGGCGGCG-CCgaaUCGagGAGCgUGCCc -3' miRNA: 3'- ggCGUCGUCGUaGG---AGCa-CUCG-AUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 44877 | 1.15 | 0.000475 |
Target: 5'- cCCGCAGCAGCAUCCUCGUGAGCUACCa -3' miRNA: 3'- -GGCGUCGUCGUAGGAGCACUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 44827 | 0.67 | 0.683965 |
Target: 5'- -gGgGGCGGCGUCauuggCGUGcccgcuGCUGCCg -3' miRNA: 3'- ggCgUCGUCGUAGga---GCACu-----CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 43131 | 0.67 | 0.671979 |
Target: 5'- cCCGCAGguuGcCGUCCUCGacggcgagcuuguUGAGCgagcGCCa -3' miRNA: 3'- -GGCGUCgu-C-GUAGGAGC-------------ACUCGa---UGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 41916 | 0.66 | 0.737471 |
Target: 5'- gUCGCAGUcgccGCAUCCg---GAGgUACCg -3' miRNA: 3'- -GGCGUCGu---CGUAGGagcaCUCgAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 38648 | 0.66 | 0.726935 |
Target: 5'- aCCGCAucacGC-GCGUgCCcuaUCGcccgGAGCUGCCg -3' miRNA: 3'- -GGCGU----CGuCGUA-GG---AGCa---CUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 37179 | 0.69 | 0.542194 |
Target: 5'- gCCGCGGCAGgAUCUugaaaUCGguacGGCUGCg -3' miRNA: 3'- -GGCGUCGUCgUAGG-----AGCac--UCGAUGg -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 35038 | 0.71 | 0.459512 |
Target: 5'- gCgGCAGCAGCggCCugaUCGUcGucGCUGCCg -3' miRNA: 3'- -GgCGUCGUCGuaGG---AGCA-Cu-CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 34990 | 0.66 | 0.7479 |
Target: 5'- uCCGagccuGCAGCGUUUgCGUuuGCUGCCg -3' miRNA: 3'- -GGCgu---CGUCGUAGGaGCAcuCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 33007 | 0.71 | 0.430251 |
Target: 5'- cCCGCAGUGGCGUCUUUGUcaauGaAGUUGCa -3' miRNA: 3'- -GGCGUCGUCGUAGGAGCA----C-UCGAUGg -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 31146 | 0.74 | 0.30194 |
Target: 5'- gCGCuGCAgGCcugCCUCGcgGAGCUGCCc -3' miRNA: 3'- gGCGuCGU-CGua-GGAGCa-CUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 30675 | 0.71 | 0.43409 |
Target: 5'- uCCG-GGCGGCAUCCUCGcucgcggcaaguaucUGAGCaugaucGCCg -3' miRNA: 3'- -GGCgUCGUCGUAGGAGC---------------ACUCGa-----UGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 28469 | 0.68 | 0.607265 |
Target: 5'- cCCGCuguugGGCGGUAUCCgUUG-GAGCUuugggacaagGCCg -3' miRNA: 3'- -GGCG-----UCGUCGUAGG-AGCaCUCGA----------UGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 26725 | 0.7 | 0.520981 |
Target: 5'- -gGCAGguGuCAcCCUCGccGGCUACCa -3' miRNA: 3'- ggCGUCguC-GUaGGAGCacUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 25294 | 0.66 | 0.746862 |
Target: 5'- aCCGuCAGuCGGCGcaggcaacuUCCUUggucucaugccugGUGAGCUGCg -3' miRNA: 3'- -GGC-GUC-GUCGU---------AGGAG-------------CACUCGAUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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