Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12403 | 5' | -55.6 | NC_003324.1 | + | 44884 | 0.7 | 0.435973 |
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Target: 5'- aAGUCAGcgGUAcGCCGggGCgCGGgGGCu -3' miRNA: 3'- -UCGGUCuaCAU-CGGCaaCG-GCCgUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 27165 | 0.71 | 0.397936 |
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Target: 5'- cGCCAGAUGcagggAGCCGca-CCGGCcACa -3' miRNA: 3'- uCGGUCUACa----UCGGCaacGGCCGuUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 42553 | 0.71 | 0.362096 |
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Target: 5'- uGCCAGucGUGcuuGCCGcaaugaccgUGCCGGCAGCc -3' miRNA: 3'- uCGGUC--UACau-CGGCa--------ACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 25711 | 0.72 | 0.328562 |
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Target: 5'- uGCguGAUGUccaaucagcAGCCG-UGCCGGCGcaGCa -3' miRNA: 3'- uCGguCUACA---------UCGGCaACGGCCGU--UG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 33453 | 0.73 | 0.297379 |
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Target: 5'- uGGCCGGA-GUugcGCgCGgaGCCGGCAACc -3' miRNA: 3'- -UCGGUCUaCAu--CG-GCaaCGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 2419 | 0.73 | 0.289951 |
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Target: 5'- cGCCGauGAUGaAGCCGccgacGCCGGCGGCu -3' miRNA: 3'- uCGGU--CUACaUCGGCaa---CGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 43022 | 0.74 | 0.254991 |
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Target: 5'- cGGCCGGuaGUGgGGCCugcaUUGCCGGUAACg -3' miRNA: 3'- -UCGGUC--UACaUCGGc---AACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 55228 | 1.09 | 0.00076 |
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Target: 5'- cAGCCAGAUGUAGCCGUUGCCGGCAACu -3' miRNA: 3'- -UCGGUCUACAUCGGCAACGGCCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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