Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12403 | 5' | -55.6 | NC_003324.1 | + | 43022 | 0.74 | 0.254991 |
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Target: 5'- cGGCCGGuaGUGgGGCCugcaUUGCCGGUAACg -3' miRNA: 3'- -UCGGUC--UACaUCGGc---AACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 44884 | 0.7 | 0.435973 |
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Target: 5'- aAGUCAGcgGUAcGCCGggGCgCGGgGGCu -3' miRNA: 3'- -UCGGUCuaCAU-CGGCaaCG-GCCgUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 51531 | 0.67 | 0.605109 |
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Target: 5'- uGGCCGGuggcuUGggcuGCCGUcuUGC-GGCAACg -3' miRNA: 3'- -UCGGUCu----ACau--CGGCA--ACGgCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 53490 | 0.67 | 0.594024 |
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Target: 5'- aAGCC-GA---GGCCGaaUGCCGGCGAUg -3' miRNA: 3'- -UCGGuCUacaUCGGCa-ACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 53720 | 0.68 | 0.528501 |
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Target: 5'- cGCaCGGcgGUAGUucuuuUGgaGCCGGCGACg -3' miRNA: 3'- uCG-GUCuaCAUCG-----GCaaCGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 55228 | 1.09 | 0.00076 |
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Target: 5'- cAGCCAGAUGUAGCCGUUGCCGGCAACu -3' miRNA: 3'- -UCGGUCUACAUCGGCAACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 56139 | 0.66 | 0.693805 |
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Target: 5'- cGCCAGccugGaAGCCGacgGCCguGGCGACu -3' miRNA: 3'- uCGGUCua--CaUCGGCaa-CGG--CCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 56821 | 0.66 | 0.638469 |
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Target: 5'- uGCCGGggGUGGUgGgauUGCgGcGCAACg -3' miRNA: 3'- uCGGUCuaCAUCGgCa--ACGgC-CGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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