Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12403 | 5' | -55.6 | NC_003324.1 | + | 27165 | 0.71 | 0.397936 |
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Target: 5'- cGCCAGAUGcagggAGCCGca-CCGGCcACa -3' miRNA: 3'- uCGGUCUACa----UCGGCaacGGCCGuUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 25711 | 0.72 | 0.328562 |
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Target: 5'- uGCguGAUGUccaaucagcAGCCG-UGCCGGCGcaGCa -3' miRNA: 3'- uCGguCUACA---------UCGGCaACGGCCGU--UG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 25309 | 0.67 | 0.627341 |
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Target: 5'- cGGCCAGGUGcucgaaccgucAGUCGgcGCaGGCAACu -3' miRNA: 3'- -UCGGUCUACa----------UCGGCaaCGgCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 17777 | 0.66 | 0.649591 |
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Target: 5'- gGGCCAucau--GCCGgugaUGCCGGCGAUc -3' miRNA: 3'- -UCGGUcuacauCGGCa---ACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 17736 | 0.67 | 0.605109 |
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Target: 5'- cAGCaGGuUGaAGCCGauugUGCCGGUGACa -3' miRNA: 3'- -UCGgUCuACaUCGGCa---ACGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 9480 | 0.66 | 0.649591 |
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Target: 5'- cGGCCcgcuaccugaAGAaugGUGGCacg-GCCGGCGGCa -3' miRNA: 3'- -UCGG----------UCUa--CAUCGgcaaCGGCCGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 5822 | 0.66 | 0.638469 |
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Target: 5'- cGCUcGAaacgGUAGCCGgUGCCGcCAACg -3' miRNA: 3'- uCGGuCUa---CAUCGGCaACGGCcGUUG- -5' |
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| 12403 | 5' | -55.6 | NC_003324.1 | + | 2419 | 0.73 | 0.289951 |
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Target: 5'- cGCCGauGAUGaAGCCGccgacGCCGGCGGCu -3' miRNA: 3'- uCGGU--CUACaUCGGCaa---CGGCCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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