Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12404 | 5' | -51.4 | NC_003324.1 | + | 30616 | 0.66 | 0.930478 |
Target: 5'- -aG-CGcgUCG-CAGCGGcAGCGGCa -3' miRNA: 3'- ggCaGUuaAGCaGUCGCUaUCGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 3396 | 0.66 | 0.930478 |
Target: 5'- aCCGUC--UUCGggcugCAGUGAaGGCGGa- -3' miRNA: 3'- -GGCAGuuAAGCa----GUCGCUaUCGCCgc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 25508 | 0.66 | 0.930478 |
Target: 5'- uUGUCGAguUUCcgcaacagGGUGAUGGCGGCGa -3' miRNA: 3'- gGCAGUU--AAGcag-----UCGCUAUCGCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 25652 | 0.66 | 0.924725 |
Target: 5'- aCCGcCGAggacaaUC-UCGGCGAaGGCGGCc -3' miRNA: 3'- -GGCaGUUa-----AGcAGUCGCUaUCGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 8326 | 0.66 | 0.924725 |
Target: 5'- gCCGUCGgcgcucguuacgGUgacCGUCGGCGGggAGCuGCGu -3' miRNA: 3'- -GGCAGU------------UAa--GCAGUCGCUa-UCGcCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 52183 | 0.67 | 0.898899 |
Target: 5'- cUCGUCAAaauaaUCGUCGGCGuccuUA-CGGCa -3' miRNA: 3'- -GGCAGUUa----AGCAGUCGCu---AUcGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 23461 | 0.67 | 0.891749 |
Target: 5'- gCGUCAAagCGgagUGGCGAaAGUGGCu -3' miRNA: 3'- gGCAGUUaaGCa--GUCGCUaUCGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 2506 | 0.68 | 0.863816 |
Target: 5'- gCCGcCGg--CGUCGGCGGcuucaucaucggcguUGGCGGUGu -3' miRNA: 3'- -GGCaGUuaaGCAGUCGCU---------------AUCGCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 29944 | 0.68 | 0.860509 |
Target: 5'- uCCGgacgggCGAggggCGUCcuGCG-UAGCGGCGg -3' miRNA: 3'- -GGCa-----GUUaa--GCAGu-CGCuAUCGCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 20423 | 0.68 | 0.860509 |
Target: 5'- aUGUCGAaccggcUUCuGUCGGCGAUGGCGa-- -3' miRNA: 3'- gGCAGUU------AAG-CAGUCGCUAUCGCcgc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 7418 | 0.68 | 0.852071 |
Target: 5'- aCCG-CA--UCGUCAGCGugcccucGGCGcGCGg -3' miRNA: 3'- -GGCaGUuaAGCAGUCGCua-----UCGC-CGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 12273 | 0.68 | 0.852071 |
Target: 5'- aCCGUCcggcggcUCGUuccaguccgCAGaCGGUAGCGGCu -3' miRNA: 3'- -GGCAGuua----AGCA---------GUC-GCUAUCGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 29137 | 0.68 | 0.834495 |
Target: 5'- uCCGUCGAggaccUCGcCAGC-AUGGCGGa- -3' miRNA: 3'- -GGCAGUUa----AGCaGUCGcUAUCGCCgc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 48279 | 0.68 | 0.834495 |
Target: 5'- cUCGUCAGUggucUCGgagaacCAGCGAacGCGGCc -3' miRNA: 3'- -GGCAGUUA----AGCa-----GUCGCUauCGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 48944 | 0.68 | 0.825376 |
Target: 5'- gCCGUCGcUUCuUCGGCaGUcGCGGCa -3' miRNA: 3'- -GGCAGUuAAGcAGUCGcUAuCGCCGc -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 44574 | 0.69 | 0.786924 |
Target: 5'- cCCGU----UCGgCAGCGAcacGGCGGCGa -3' miRNA: 3'- -GGCAguuaAGCaGUCGCUa--UCGCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 19366 | 0.69 | 0.786924 |
Target: 5'- cUCGUC----CGUCAGCGAUGGaucgacgcCGGCGc -3' miRNA: 3'- -GGCAGuuaaGCAGUCGCUAUC--------GCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 29796 | 0.69 | 0.776871 |
Target: 5'- aUCGUU--UUCGaCAGCGGUAcCGGCGa -3' miRNA: 3'- -GGCAGuuAAGCaGUCGCUAUcGCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 21388 | 0.7 | 0.766665 |
Target: 5'- aUCGagGAUaUCGcCGGCGA-GGCGGCGa -3' miRNA: 3'- -GGCagUUA-AGCaGUCGCUaUCGCCGC- -5' |
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12404 | 5' | -51.4 | NC_003324.1 | + | 30327 | 0.7 | 0.755275 |
Target: 5'- aUCGcCGAUggUGUCGGCGGUGGCgucgcucGGCGc -3' miRNA: 3'- -GGCaGUUAa-GCAGUCGCUAUCG-------CCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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