Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12404 | 5' | -51.4 | NC_003324.1 | + | 2609 | 0.7 | 0.745839 |
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Target: 5'- uCCGUCGccuGUUCGUCGGUGGcgucaCGGCu -3' miRNA: 3'- -GGCAGU---UAAGCAGUCGCUauc--GCCGc -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 18947 | 0.7 | 0.735244 |
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Target: 5'- aCCGUCuauauccCGUCcguGGCGAagUAGCGGCc -3' miRNA: 3'- -GGCAGuuaa---GCAG---UCGCU--AUCGCCGc -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 35448 | 0.72 | 0.636515 |
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Target: 5'- uUCGUgCAGgaucaCGUCGGCGAUGuCGGCGa -3' miRNA: 3'- -GGCA-GUUaa---GCAGUCGCUAUcGCCGC- -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 41061 | 0.72 | 0.603137 |
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Target: 5'- gCCuugCAGcgCGUCAccGUGAUAGCGGCGa -3' miRNA: 3'- -GGca-GUUaaGCAGU--CGCUAUCGCCGC- -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 20785 | 0.72 | 0.603137 |
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Target: 5'- aCCGcCGGaaCGUUGGCGA-GGCGGCGg -3' miRNA: 3'- -GGCaGUUaaGCAGUCGCUaUCGCCGC- -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 9297 | 0.72 | 0.603137 |
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Target: 5'- aCCGagAccUCGUCAuCGGUGGCGGCa -3' miRNA: 3'- -GGCagUuaAGCAGUcGCUAUCGCCGc -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 3460 | 0.74 | 0.515868 |
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Target: 5'- gCGUCAcgUCGauUCGGUaAUGGCGGCa -3' miRNA: 3'- gGCAGUuaAGC--AGUCGcUAUCGCCGc -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 18352 | 0.77 | 0.378 |
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Target: 5'- gCGUCGgccgAUUcCGUCAGCGGUcGCGGCu -3' miRNA: 3'- gGCAGU----UAA-GCAGUCGCUAuCGCCGc -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 13180 | 0.79 | 0.267136 |
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Target: 5'- aCCGUCAGcgaggcugCGUCGGCGGcggucGGCGGCGg -3' miRNA: 3'- -GGCAGUUaa------GCAGUCGCUa----UCGCCGC- -5' |
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| 12404 | 5' | -51.4 | NC_003324.1 | + | 56669 | 1.11 | 0.002303 |
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Target: 5'- gCCGUCAAUUCGUCAGCGAUAGCGGCGu -3' miRNA: 3'- -GGCAGUUAAGCAGUCGCUAUCGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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