miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12409 5' -51.3 NC_003324.1 + 8426 0.66 0.919165
Target:  5'- uUCCGCCGGCUgCAGGUgcaGGCggauuuGCGg -3'
miRNA:   3'- -GGGUGGUUGAaGUUCAa--CCGgau---CGC- -5'
12409 5' -51.3 NC_003324.1 + 48365 0.66 0.891905
Target:  5'- cUCCaaGCCGGCUUCGcAGUUGcCCUuGGCGc -3'
miRNA:   3'- -GGG--UGGUUGAAGU-UCAACcGGA-UCGC- -5'
12409 5' -51.3 NC_003324.1 + 42764 0.67 0.868539
Target:  5'- cCUCGCCAGCgguugCcGGUccuggUGGCCcGGCGa -3'
miRNA:   3'- -GGGUGGUUGaa---GuUCA-----ACCGGaUCGC- -5'
12409 5' -51.3 NC_003324.1 + 52291 0.68 0.842851
Target:  5'- aCCGCCcgaUUCGGGgcGGCC-GGCGu -3'
miRNA:   3'- gGGUGGuugAAGUUCaaCCGGaUCGC- -5'
12409 5' -51.3 NC_003324.1 + 26278 0.68 0.824547
Target:  5'- aCCCGCUcuCUUCGAGcUGGCUcacuucGCGg -3'
miRNA:   3'- -GGGUGGuuGAAGUUCaACCGGau----CGC- -5'
12409 5' -51.3 NC_003324.1 + 57062 0.71 0.666548
Target:  5'- uCCgCAUCAACUaugcUCGGGUUGGgCUGGgGa -3'
miRNA:   3'- -GG-GUGGUUGA----AGUUCAACCgGAUCgC- -5'
12409 5' -51.3 NC_003324.1 + 45429 0.71 0.644016
Target:  5'- gCCGCCuGCUUCAAGUcgcccccuaaaUGGUUgAGCGa -3'
miRNA:   3'- gGGUGGuUGAAGUUCA-----------ACCGGaUCGC- -5'
12409 5' -51.3 NC_003324.1 + 35832 0.72 0.610158
Target:  5'- gCCCAgCAGCUccaUCAGGgauucgaccUGcGCCUGGCGa -3'
miRNA:   3'- -GGGUgGUUGA---AGUUCa--------AC-CGGAUCGC- -5'
12409 5' -51.3 NC_003324.1 + 9561 0.72 0.587668
Target:  5'- gCCACCAuuCUUCAGGUagcgGGCCgauccAGCa -3'
miRNA:   3'- gGGUGGUu-GAAGUUCAa---CCGGa----UCGc -5'
12409 5' -51.3 NC_003324.1 + 19557 0.72 0.587668
Target:  5'- aCUCGCCGACggcggUCAuGUUGGaCCgGGCGa -3'
miRNA:   3'- -GGGUGGUUGa----AGUuCAACC-GGaUCGC- -5'
12409 5' -51.3 NC_003324.1 + 4764 0.74 0.489365
Target:  5'- -aCGCCAuCUUCAAGggUGGCCUuGGCa -3'
miRNA:   3'- ggGUGGUuGAAGUUCa-ACCGGA-UCGc -5'
12409 5' -51.3 NC_003324.1 + 2455 1.11 0.002031
Target:  5'- cCCCACCAACUUCAAGUUGGCCUAGCGc -3'
miRNA:   3'- -GGGUGGUUGAAGUUCAACCGGAUCGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.