Results 21 - 35 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12415 | 3' | -50.9 | NC_003324.1 | + | 13101 | 0.69 | 0.773588 |
Target: 5'- uGAGAGgGCgGGCGuUGAUGCCGCc-- -3' miRNA: 3'- cUUCUUgCGgUUGU-ACUACGGCGacu -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 7166 | 0.69 | 0.783974 |
Target: 5'- ----cGCGCCAACAguUGGUG-CGCUGGc -3' miRNA: 3'- cuucuUGCGGUUGU--ACUACgGCGACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 14608 | 0.67 | 0.842289 |
Target: 5'- --uGAGCGCCAGCGccuUGUCGCgGAc -3' miRNA: 3'- cuuCUUGCGGUUGUacuACGGCGaCU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 23233 | 0.67 | 0.842289 |
Target: 5'- --uGGGCGCCGGCAaGAcguuUGCCGCc-- -3' miRNA: 3'- cuuCUUGCGGUUGUaCU----ACGGCGacu -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 49842 | 0.67 | 0.851234 |
Target: 5'- cGAAGAcugcagcguuccACGUgAugGUGGUGCgccCGCUGAa -3' miRNA: 3'- -CUUCU------------UGCGgUugUACUACG---GCGACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 43009 | 0.67 | 0.859073 |
Target: 5'- cGAGAcCGCCGGCGcagugaccgccaaUGGUcgccccGCCGCUGGg -3' miRNA: 3'- cUUCUuGCGGUUGU-------------ACUA------CGGCGACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 50046 | 0.67 | 0.859931 |
Target: 5'- aGGGcAGCGCCGAucgcCAUGcugacgagcGUGCUGCUGAu -3' miRNA: 3'- cUUC-UUGCGGUU----GUAC---------UACGGCGACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 27757 | 0.67 | 0.86837 |
Target: 5'- aGGAGAagcucgGCGCCAcGCAUGAggugGUCGCg-- -3' miRNA: 3'- -CUUCU------UGCGGU-UGUACUa---CGGCGacu -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 35727 | 0.66 | 0.891316 |
Target: 5'- aGAAGGAgGCCGaggccaaacagcaGCAgaUGAUGCUGCg-- -3' miRNA: 3'- -CUUCUUgCGGU-------------UGU--ACUACGGCGacu -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 10288 | 0.66 | 0.892065 |
Target: 5'- ----uGCGCCGGCAUGAUauuggcGCCGaUGAg -3' miRNA: 3'- cuucuUGCGGUUGUACUA------CGGCgACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 15047 | 0.66 | 0.892065 |
Target: 5'- -----gUGCCGACGUGAUGcCCGCc-- -3' miRNA: 3'- cuucuuGCGGUUGUACUAC-GGCGacu -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 50279 | 0.66 | 0.899401 |
Target: 5'- aAGGcuCGCCGACu---UGUCGCUGAu -3' miRNA: 3'- cUUCuuGCGGUUGuacuACGGCGACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 28971 | 0.66 | 0.899401 |
Target: 5'- cGAAGGACgagGCCGGCcaGUGGUGggaCGUUGAg -3' miRNA: 3'- -CUUCUUG---CGGUUG--UACUACg--GCGACU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 14519 | 0.66 | 0.906446 |
Target: 5'- --uGGACGCCGGCAUGG-GCC-CaGAg -3' miRNA: 3'- cuuCUUGCGGUUGUACUaCGGcGaCU- -5' |
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12415 | 3' | -50.9 | NC_003324.1 | + | 24721 | 0.66 | 0.913197 |
Target: 5'- cGAcaAGC-CCGACAUGAUGCuCGgUGAc -3' miRNA: 3'- -CUucUUGcGGUUGUACUACG-GCgACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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