Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12416 | 5' | -58.4 | NC_003324.1 | + | 5456 | 0.66 | 0.555652 |
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Target: 5'- --cGCUggGUCAGGCaGGCGgcGUCGCc -3' miRNA: 3'- accCGG--CAGUCCGaCCGCaaUAGCGa -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 10243 | 0.67 | 0.463183 |
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Target: 5'- cGGGCCGagAGGUcgGGCaUUGucUCGCg -3' miRNA: 3'- aCCCGGCagUCCGa-CCGcAAU--AGCGa -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 28192 | 0.68 | 0.406213 |
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Target: 5'- gUGGGC--UCGGGCagGGCGUUagacggcgaaGUCGCg -3' miRNA: 3'- -ACCCGgcAGUCCGa-CCGCAA----------UAGCGa -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 7653 | 0.69 | 0.388223 |
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Target: 5'- cGcGCCGgcagugCcGGCUGGCGUuuaugUGUCGCUg -3' miRNA: 3'- aCcCGGCa-----GuCCGACCGCA-----AUAGCGA- -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 21109 | 0.7 | 0.329515 |
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Target: 5'- -cGGCuuccaCGUCGGGCUGGCGcUUG-CGCa -3' miRNA: 3'- acCCG-----GCAGUCCGACCGC-AAUaGCGa -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 56059 | 0.7 | 0.328725 |
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Target: 5'- -cGGCCGUCggcuuccAGGCUGGCGaaccCGCg -3' miRNA: 3'- acCCGGCAG-------UCCGACCGCaauaGCGa -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 49321 | 0.71 | 0.264045 |
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Target: 5'- cGGGcCCGUCGGGCugcccgaucaUGGCG---UCGCa -3' miRNA: 3'- aCCC-GGCAGUCCG----------ACCGCaauAGCGa -5' |
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| 12416 | 5' | -58.4 | NC_003324.1 | + | 4886 | 1.07 | 0.000646 |
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Target: 5'- aUGGGCCGUCAGGCUGGCGUUAUCGCUu -3' miRNA: 3'- -ACCCGGCAGUCCGACCGCAAUAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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