Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12419 | 3' | -54.3 | NC_003324.1 | + | 19284 | 0.67 | 0.73747 |
Target: 5'- -uGCCGAacAACUcCCGgcccuugGGCGGCAUa -3' miRNA: 3'- guUGGCU--UUGGaGGCaa-----CCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 32298 | 0.67 | 0.705593 |
Target: 5'- gCGACaacGGCCUCUGc--GCGGCGCCg -3' miRNA: 3'- -GUUGgcuUUGGAGGCaacCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 1062 | 0.68 | 0.683965 |
Target: 5'- -uACCGgcACCaagUCC--UGGCGGCugCg -3' miRNA: 3'- guUGGCuuUGG---AGGcaACCGCCGugG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 40538 | 0.68 | 0.651179 |
Target: 5'- gCGGCgaGAAGCCUCUGgUGGUuuucgaucGGCACUa -3' miRNA: 3'- -GUUGg-CUUUGGAGGCaACCG--------CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 49595 | 0.69 | 0.618235 |
Target: 5'- aGGCUGAccaGACCUauggcgauaCCGUcauucUGGCaGGCGCCa -3' miRNA: 3'- gUUGGCU---UUGGA---------GGCA-----ACCG-CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 5723 | 1.12 | 0.000837 |
Target: 5'- gCAACCGAAACCUCCGUUGGCGGCACCg -3' miRNA: 3'- -GUUGGCUUUGGAGGCAACCGCCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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