Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12419 | 3' | -54.3 | NC_003324.1 | + | 33780 | 0.66 | 0.788335 |
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Target: 5'- -cACCGAAggauuggaaGCCUgUGaugccUUGGCGGCAgCg -3' miRNA: 3'- guUGGCUU---------UGGAgGC-----AACCGCCGUgG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 23628 | 0.66 | 0.788335 |
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Target: 5'- -cGCCGAGcgcGCCcgCCGccacgcugUGG-GGCACCg -3' miRNA: 3'- guUGGCUU---UGGa-GGCa-------ACCgCCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 42389 | 0.66 | 0.798066 |
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Target: 5'- -cACCGgcGCCcggCaCGUggcaGGCGGCGCa -3' miRNA: 3'- guUGGCuuUGGa--G-GCAa---CCGCCGUGg -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 44436 | 0.66 | 0.798066 |
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Target: 5'- -cGCCGAGACUUCgGUcgcugaUGGcCGGCGu- -3' miRNA: 3'- guUGGCUUUGGAGgCA------ACC-GCCGUgg -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 30684 | 0.66 | 0.807625 |
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Target: 5'- -cACCau-GCUUCCG--GGCGGCAUCc -3' miRNA: 3'- guUGGcuuUGGAGGCaaCCGCCGUGG- -5' |
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| 12419 | 3' | -54.3 | NC_003324.1 | + | 20787 | 0.66 | 0.807625 |
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Target: 5'- -cGCCGGAACgUUgGcgaGGCGGCggACCg -3' miRNA: 3'- guUGGCUUUGgAGgCaa-CCGCCG--UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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