Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12419 | 3' | -54.3 | NC_003324.1 | + | 21853 | 0.73 | 0.384016 |
Target: 5'- gCAGCCG-GGCCUCCuc-GGCGGCcuugGCCu -3' miRNA: 3'- -GUUGGCuUUGGAGGcaaCCGCCG----UGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 14810 | 0.74 | 0.365605 |
Target: 5'- gCGGCCGGcaucggcAGCUUCCug-GGCGGCAUCg -3' miRNA: 3'- -GUUGGCU-------UUGGAGGcaaCCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 30483 | 0.77 | 0.238986 |
Target: 5'- cCAAUCgGAAGCCUCCGUauccuggucgagGGCGGCAUg -3' miRNA: 3'- -GUUGG-CUUUGGAGGCAa-----------CCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 27262 | 0.79 | 0.179481 |
Target: 5'- gCGGCCGAAcacgGCUUCCGcgUUGGUGGCGCg -3' miRNA: 3'- -GUUGGCUU----UGGAGGC--AACCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 9296 | 0.86 | 0.058154 |
Target: 5'- -uACCGAGACCUCgucauCGgUGGCGGCACCa -3' miRNA: 3'- guUGGCUUUGGAG-----GCaACCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 5723 | 1.12 | 0.000837 |
Target: 5'- gCAACCGAAACCUCCGUUGGCGGCACCg -3' miRNA: 3'- -GUUGGCUUUGGAGGCAACCGCCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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