Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12419 | 3' | -54.3 | NC_003324.1 | + | 40538 | 0.68 | 0.651179 |
Target: 5'- gCGGCgaGAAGCCUCUGgUGGUuuucgaucGGCACUa -3' miRNA: 3'- -GUUGg-CUUUGGAGGCaACCG--------CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 39822 | 0.68 | 0.673071 |
Target: 5'- -cGCCGGAcACCaaggCCGccaugUGGUGGCugCa -3' miRNA: 3'- guUGGCUU-UGGa---GGCa----ACCGCCGugG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 1062 | 0.68 | 0.683965 |
Target: 5'- -uACCGgcACCaagUCC--UGGCGGCugCg -3' miRNA: 3'- guUGGCuuUGG---AGGcaACCGCCGugG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 32298 | 0.67 | 0.705593 |
Target: 5'- gCGACaacGGCCUCUGc--GCGGCGCCg -3' miRNA: 3'- -GUUGgcuUUGGAGGCaacCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 39265 | 0.67 | 0.705593 |
Target: 5'- uGAUCGGuuGCCgCCG-UGGCuGCGCCa -3' miRNA: 3'- gUUGGCUu-UGGaGGCaACCGcCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 49588 | 0.67 | 0.713099 |
Target: 5'- aCGGCCGAGagugccagaaugccGCCcUCGUUGcggacCGGCACCu -3' miRNA: 3'- -GUUGGCUU--------------UGGaGGCAACc----GCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 28497 | 0.67 | 0.715238 |
Target: 5'- aCAGCCGAGACauccccaUCCauccgagcccgcuGUUgGGCGGUAUCc -3' miRNA: 3'- -GUUGGCUUUGg------AGG-------------CAA-CCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 20707 | 0.67 | 0.716305 |
Target: 5'- aAGCCaaagggcaaGGAGCCUaCCGUcgaagucacUGGCgacGGCGCCa -3' miRNA: 3'- gUUGG---------CUUUGGA-GGCA---------ACCG---CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 45995 | 0.67 | 0.716305 |
Target: 5'- -uGCUuc-GCUUCCGaagcGGCGGCGCCg -3' miRNA: 3'- guUGGcuuUGGAGGCaa--CCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 34068 | 0.67 | 0.716305 |
Target: 5'- cCGACCGAAucgggcuccGCCuuUCCGUcgGGCgGGUGCUu -3' miRNA: 3'- -GUUGGCUU---------UGG--AGGCAa-CCG-CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 19284 | 0.67 | 0.73747 |
Target: 5'- -uGCCGAacAACUcCCGgcccuugGGCGGCAUa -3' miRNA: 3'- guUGGCU--UUGGaGGCaa-----CCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 42005 | 0.67 | 0.73747 |
Target: 5'- gAACgCGguACCUCCGgauGCGGCgACUg -3' miRNA: 3'- gUUG-GCuuUGGAGGCaacCGCCG-UGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 54877 | 0.67 | 0.7479 |
Target: 5'- --cCCGGAugCUCgCGaUGuCGGCGCCc -3' miRNA: 3'- guuGGCUUugGAG-GCaACcGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 29315 | 0.67 | 0.758212 |
Target: 5'- --cCCGAAcaGCCgCCGUcuUGGUGagcGCACCg -3' miRNA: 3'- guuGGCUU--UGGaGGCA--ACCGC---CGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 25011 | 0.67 | 0.758212 |
Target: 5'- uCGAUCGAGACaCUgCGaccgGGCGGCguGCUg -3' miRNA: 3'- -GUUGGCUUUG-GAgGCaa--CCGCCG--UGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 53643 | 0.67 | 0.758212 |
Target: 5'- cCAAaaGAAcuACCgCCGU--GCGGCACCu -3' miRNA: 3'- -GUUggCUU--UGGaGGCAacCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 28538 | 0.66 | 0.768397 |
Target: 5'- -cACUGAGGCCUgCCGcgcucaacaUGGCGGCGu- -3' miRNA: 3'- guUGGCUUUGGA-GGCa--------ACCGCCGUgg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 27687 | 0.66 | 0.768397 |
Target: 5'- -cGCCGAGcuUCUCC-UUGGCGauguGCGCCu -3' miRNA: 3'- guUGGCUUu-GGAGGcAACCGC----CGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 4932 | 0.66 | 0.778441 |
Target: 5'- uCGACCG--GCCgcaaCGUUGuugcCGGCGCCa -3' miRNA: 3'- -GUUGGCuuUGGag--GCAACc---GCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 26682 | 0.66 | 0.778441 |
Target: 5'- gGAUCGAuGCCgCUGUUGaGCGuGCuACCg -3' miRNA: 3'- gUUGGCUuUGGaGGCAAC-CGC-CG-UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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