Results 21 - 40 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12419 | 3' | -54.3 | NC_003324.1 | + | 29315 | 0.67 | 0.758212 |
Target: 5'- --cCCGAAcaGCCgCCGUcuUGGUGagcGCACCg -3' miRNA: 3'- guuGGCUU--UGGaGGCA--ACCGC---CGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 30483 | 0.77 | 0.238986 |
Target: 5'- cCAAUCgGAAGCCUCCGUauccuggucgagGGCGGCAUg -3' miRNA: 3'- -GUUGG-CUUUGGAGGCAa-----------CCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 30563 | 0.69 | 0.629217 |
Target: 5'- --uCCGAgGGCCUgCuc-GGCGGCGCCg -3' miRNA: 3'- guuGGCU-UUGGAgGcaaCCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 30684 | 0.66 | 0.807625 |
Target: 5'- -cACCau-GCUUCCG--GGCGGCAUCc -3' miRNA: 3'- guUGGcuuUGGAGGCaaCCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 32298 | 0.67 | 0.705593 |
Target: 5'- gCGACaacGGCCUCUGc--GCGGCGCCg -3' miRNA: 3'- -GUUGgcuUUGGAGGCaacCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 33780 | 0.66 | 0.788335 |
Target: 5'- -cACCGAAggauuggaaGCCUgUGaugccUUGGCGGCAgCg -3' miRNA: 3'- guUGGCUU---------UGGAgGC-----AACCGCCGUgG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 34068 | 0.67 | 0.716305 |
Target: 5'- cCGACCGAAucgggcuccGCCuuUCCGUcgGGCgGGUGCUu -3' miRNA: 3'- -GUUGGCUU---------UGG--AGGCAa-CCG-CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 35654 | 0.72 | 0.430251 |
Target: 5'- uGGCCucGGCCUCCuucUUGGCGGCuuCCu -3' miRNA: 3'- gUUGGcuUUGGAGGc--AACCGCCGu-GG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 36749 | 0.69 | 0.595223 |
Target: 5'- -uACCGGAcaagacaucucgcAUCUCC---GGCGGCACCu -3' miRNA: 3'- guUGGCUU-------------UGGAGGcaaCCGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 37467 | 0.69 | 0.617137 |
Target: 5'- gAGCCGGggUCUCCGggaugGGCGacugguaGCGCUu -3' miRNA: 3'- gUUGGCUuuGGAGGCaa---CCGC-------CGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 39265 | 0.67 | 0.705593 |
Target: 5'- uGAUCGGuuGCCgCCG-UGGCuGCGCCa -3' miRNA: 3'- gUUGGCUu-UGGaGGCaACCGcCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 39822 | 0.68 | 0.673071 |
Target: 5'- -cGCCGGAcACCaaggCCGccaugUGGUGGCugCa -3' miRNA: 3'- guUGGCUU-UGGa---GGCa----ACCGCCGugG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 40535 | 0.69 | 0.629217 |
Target: 5'- aAGCgGAGACa-CUGaUGGCGGCACg -3' miRNA: 3'- gUUGgCUUUGgaGGCaACCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 40538 | 0.68 | 0.651179 |
Target: 5'- gCGGCgaGAAGCCUCUGgUGGUuuucgaucGGCACUa -3' miRNA: 3'- -GUUGg-CUUUGGAGGCaACCG--------CCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 42005 | 0.67 | 0.73747 |
Target: 5'- gAACgCGguACCUCCGgauGCGGCgACUg -3' miRNA: 3'- gUUG-GCuuUGGAGGCaacCGCCG-UGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 42389 | 0.66 | 0.798066 |
Target: 5'- -cACCGgcGCCcggCaCGUggcaGGCGGCGCa -3' miRNA: 3'- guUGGCuuUGGa--G-GCAa---CCGCCGUGg -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 42909 | 0.69 | 0.585398 |
Target: 5'- cCAGCgCGggGCgaCCaUUGGCGGuCACUg -3' miRNA: 3'- -GUUG-GCuuUGgaGGcAACCGCC-GUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 43896 | 0.7 | 0.574518 |
Target: 5'- gAGCCGAucccuuguugAGCCUuuGgcGcCGGCACCg -3' miRNA: 3'- gUUGGCU----------UUGGAggCaaCcGCCGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 44322 | 0.71 | 0.479595 |
Target: 5'- gCGGCCGAuAGCCUCUGcagccacGGCGcGCGCUg -3' miRNA: 3'- -GUUGGCU-UUGGAGGCaa-----CCGC-CGUGG- -5' |
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12419 | 3' | -54.3 | NC_003324.1 | + | 44436 | 0.66 | 0.798066 |
Target: 5'- -cGCCGAGACUUCgGUcgcugaUGGcCGGCGu- -3' miRNA: 3'- guUGGCUUUGGAGgCA------ACC-GCCGUgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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