Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12421 | 3' | -55.5 | NC_003324.1 | + | 33049 | 0.68 | 0.530376 |
Target: 5'- -gCGACGGCGA-GCAGa--UGCAGCg -3' miRNA: 3'- uaGCUGCCGUUcCGUUgugACGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 42201 | 0.67 | 0.563151 |
Target: 5'- -gCGGCGGCAuuGGCGACGagUGCgaAGCg -3' miRNA: 3'- uaGCUGCCGUu-CCGUUGUg-ACG--UCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 36503 | 0.67 | 0.574202 |
Target: 5'- -aUGGCGGUAGGGCGACGgcGaCAGCc -3' miRNA: 3'- uaGCUGCCGUUCCGUUGUgaC-GUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 38845 | 0.67 | 0.574202 |
Target: 5'- cGUCGGCGucccaccagcucGCGaaaugcAGGCGAUACUGCAgGCg -3' miRNA: 3'- -UAGCUGC------------CGU------UCCGUUGUGACGU-CGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 14598 | 0.67 | 0.607614 |
Target: 5'- -cCGGCGGCGcAGGCGguaGCgugGCGGCc -3' miRNA: 3'- uaGCUGCCGU-UCCGUug-UGa--CGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 18713 | 0.67 | 0.62329 |
Target: 5'- cAUCGACu-CGAGGUAACGCUGCcauaccugauccaucAGCUc -3' miRNA: 3'- -UAGCUGccGUUCCGUUGUGACG---------------UCGA- -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 47652 | 0.67 | 0.573094 |
Target: 5'- cUCGACGGCGAGGgucgaucggcccuCGucauccaccaugGCACUGCcGCa -3' miRNA: 3'- uAGCUGCCGUUCC-------------GU------------UGUGACGuCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 48575 | 0.67 | 0.574202 |
Target: 5'- -aUGAgGGCAAGGaCAGCGgCgucGCAGCg -3' miRNA: 3'- uaGCUgCCGUUCC-GUUGU-Ga--CGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 18518 | 0.67 | 0.607614 |
Target: 5'- -cCGGCGGCAAGGUcGCGCagaccagacCAGCg -3' miRNA: 3'- uaGCUGCCGUUCCGuUGUGac-------GUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 30363 | 0.67 | 0.618808 |
Target: 5'- uUCGGCGGCuuGGGCGACcgUGCcuuGGCc -3' miRNA: 3'- uAGCUGCCGu-UCCGUUGugACG---UCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 47834 | 0.66 | 0.652422 |
Target: 5'- -cUGACGcCuuauGAGGCGGCGCUGCcGCUg -3' miRNA: 3'- uaGCUGCcG----UUCCGUUGUGACGuCGA- -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 22593 | 0.66 | 0.641223 |
Target: 5'- gAUCGGCGGCcuuuuGGCuGC-CUGCcGCg -3' miRNA: 3'- -UAGCUGCCGuu---CCGuUGuGACGuCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 31247 | 0.66 | 0.634498 |
Target: 5'- -cUGAUGGCGcaggccGGGCAGCuccgcgaggcaggcCUGCAGCg -3' miRNA: 3'- uaGCUGCCGU------UCCGUUGu-------------GACGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 26922 | 0.66 | 0.651303 |
Target: 5'- -aUGGCGGCGaucgacgAGGCAcucaGCGgaGCGGCg -3' miRNA: 3'- uaGCUGCCGU-------UCCGU----UGUgaCGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 7543 | 0.66 | 0.652422 |
Target: 5'- gGUCGGgauuuUGGCucucGGGCAGCACagcacucggGCAGCa -3' miRNA: 3'- -UAGCU-----GCCGu---UCCGUUGUGa--------CGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 3165 | 0.66 | 0.663602 |
Target: 5'- cUCGaAUGGCAAGaGCGggaGCGCcgUGCGGCc -3' miRNA: 3'- uAGC-UGCCGUUC-CGU---UGUG--ACGUCGa -5' |
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12421 | 3' | -55.5 | NC_003324.1 | + | 39376 | 0.66 | 0.674751 |
Target: 5'- cAUC--CGGCGAaGCAgcuuGCGCUGCGGCUc -3' miRNA: 3'- -UAGcuGCCGUUcCGU----UGUGACGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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