Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12423 | 5' | -59.9 | NC_003324.1 | + | 35392 | 0.66 | 0.473588 |
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Target: 5'- --aGCAGCCGGCGcaAUCCGCcGGc- -3' miRNA: 3'- cagCGUCGGUCGCagUAGGCGuCCca -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 41915 | 0.66 | 0.454036 |
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Target: 5'- cGUCGCAGUCGcCG-CAUCCGgAGGu- -3' miRNA: 3'- -CAGCGUCGGUcGCaGUAGGCgUCCca -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 8497 | 0.67 | 0.372782 |
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Target: 5'- -cUGCAGCCGGCGgaaaucccugacccgCAUCaCGCGGGu- -3' miRNA: 3'- caGCGUCGGUCGCa--------------GUAG-GCGUCCca -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 18508 | 0.68 | 0.338023 |
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Target: 5'- gGUCGCgcagaccAGaCCAGCGUCGccuucgugugguUCCucGCAGGGUc -3' miRNA: 3'- -CAGCG-------UC-GGUCGCAGU------------AGG--CGUCCCA- -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 47906 | 0.69 | 0.300527 |
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Target: 5'- -gCGCcgauGGCCGGC-UUAUCCGCuGGGUc -3' miRNA: 3'- caGCG----UCGGUCGcAGUAGGCGuCCCA- -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 30265 | 0.7 | 0.269667 |
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Target: 5'- cGUUGCAGagGGCGUCuucgaagaauugCCGCAGGGc -3' miRNA: 3'- -CAGCGUCggUCGCAGua----------GGCGUCCCa -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 34483 | 0.7 | 0.259096 |
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Target: 5'- cGUCGUcuuguuGGCCGGCGUCuuGUUCGUcGGGUc -3' miRNA: 3'- -CAGCG------UCGGUCGCAG--UAGGCGuCCCA- -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 29810 | 0.7 | 0.252661 |
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Target: 5'- aGUCGC-GCCaacuucugGGCGaUCAUCUGCAGGcGUg -3' miRNA: 3'- -CAGCGuCGG--------UCGC-AGUAGGCGUCC-CA- -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 12999 | 0.74 | 0.130772 |
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Target: 5'- uUCGCGGCUGGCG--AUCUGCAGGGc -3' miRNA: 3'- cAGCGUCGGUCGCagUAGGCGUCCCa -5' |
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| 12423 | 5' | -59.9 | NC_003324.1 | + | 8296 | 1.07 | 0.000427 |
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Target: 5'- cGUCGCAGCCAGCGUCAUCCGCAGGGUa -3' miRNA: 3'- -CAGCGUCGGUCGCAGUAGGCGUCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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