Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12424 | 3' | -55.5 | NC_003324.1 | + | 20259 | 0.66 | 0.677229 |
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Target: 5'- cGCcUCUCUGACcgagGCUGCCGgCAgcguucuGGAUCg -3' miRNA: 3'- -UGaAGAGGCUG----UGGCGGCgGU-------UCUAG- -5' |
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| 12424 | 3' | -55.5 | NC_003324.1 | + | 35538 | 0.66 | 0.678322 |
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Target: 5'- cGCUcg-CCGACAUCGCCGaCGuGAUCc -3' miRNA: 3'- -UGAagaGGCUGUGGCGGCgGUuCUAG- -5' |
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| 12424 | 3' | -55.5 | NC_003324.1 | + | 43708 | 0.66 | 0.700055 |
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Target: 5'- --gUCgUCCGGUACCGCCugcuGCCAaucgGGAUCa -3' miRNA: 3'- ugaAG-AGGCUGUGGCGG----CGGU----UCUAG- -5' |
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| 12424 | 3' | -55.5 | NC_003324.1 | + | 32574 | 0.66 | 0.710829 |
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Target: 5'- gACaUCgUCGACACCGUgauCGCCGAGGc- -3' miRNA: 3'- -UGaAGaGGCUGUGGCG---GCGGUUCUag -5' |
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| 12424 | 3' | -55.5 | NC_003324.1 | + | 48423 | 0.66 | 0.710829 |
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Target: 5'- cGCUUCaCCGccucaucgaagaGCGCCG-CGCCGAGAa- -3' miRNA: 3'- -UGAAGaGGC------------UGUGGCgGCGGUUCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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