Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12426 | 3' | -58.8 | NC_003324.1 | + | 35883 | 0.66 | 0.581039 |
Target: 5'- uGGCu-CCGgucaaCCCCCAGaUCGCCau-CGc -3' miRNA: 3'- -CCGuuGGCa----GGGGGUC-AGCGGguuGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 8168 | 0.66 | 0.576833 |
Target: 5'- cGGCGACCGUggcagccauggucaCCUCCucgacgaAGUUGCCCu-CGu -3' miRNA: 3'- -CCGUUGGCA--------------GGGGG-------UCAGCGGGuuGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 7345 | 0.66 | 0.570536 |
Target: 5'- aGCGACCGUgaUCUCGGUCGaaguuuCCCAGCc -3' miRNA: 3'- cCGUUGGCAg-GGGGUCAGC------GGGUUGc -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 47635 | 0.66 | 0.570536 |
Target: 5'- cGGCGACCGggCCgCCA-UCGUCgAGCu -3' miRNA: 3'- -CCGUUGGCa-GGgGGUcAGCGGgUUGc -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 43244 | 0.66 | 0.570536 |
Target: 5'- aGGgGGCCG-CCUgCagGGUCGCCUGGCa -3' miRNA: 3'- -CCgUUGGCaGGGgG--UCAGCGGGUUGc -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 32896 | 0.66 | 0.56008 |
Target: 5'- aGGCGGCUG-CCCgCCAugCGCCgAACa -3' miRNA: 3'- -CCGUUGGCaGGG-GGUcaGCGGgUUGc -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 23153 | 0.66 | 0.549677 |
Target: 5'- cGGCAAaCGUCUUgCCGG-CGCCCA-CGg -3' miRNA: 3'- -CCGUUgGCAGGG-GGUCaGCGGGUuGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 15649 | 0.66 | 0.539335 |
Target: 5'- uGGCGACgG--CCCCGGcgCGCUCGAUGa -3' miRNA: 3'- -CCGUUGgCagGGGGUCa-GCGGGUUGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 25464 | 0.66 | 0.539335 |
Target: 5'- uGGCGAgcaugcuuugcUCGU-CCCgGGUCGCCgGGCGc -3' miRNA: 3'- -CCGUU-----------GGCAgGGGgUCAGCGGgUUGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 2626 | 0.67 | 0.52906 |
Target: 5'- cGGCGuGCCGUCUgCCGuccGUCGCCUguucgucgguGGCGu -3' miRNA: 3'- -CCGU-UGGCAGGgGGU---CAGCGGG----------UUGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 41440 | 0.67 | 0.518858 |
Target: 5'- cGGCuacuucauuaAGCCG-CCCCCAG-CGCCgAuuuCGa -3' miRNA: 3'- -CCG----------UUGGCaGGGGGUCaGCGGgUu--GC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 29506 | 0.67 | 0.508735 |
Target: 5'- uGGCGACCGgUUCCCGG-CGCgCAuCGa -3' miRNA: 3'- -CCGUUGGCaGGGGGUCaGCGgGUuGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 28602 | 0.67 | 0.488745 |
Target: 5'- cGGCuuCCG-CCCgCuGUCGCCCu-CGu -3' miRNA: 3'- -CCGuuGGCaGGGgGuCAGCGGGuuGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 34401 | 0.68 | 0.469132 |
Target: 5'- aGGCGaacucgccGCCGUCCUCgGcaucggugauGUCGgCCGACGg -3' miRNA: 3'- -CCGU--------UGGCAGGGGgU----------CAGCgGGUUGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 2443 | 0.68 | 0.469132 |
Target: 5'- cGGCGGCuUGUUCCCCaccaacuucaAGUUGgCCUAGCGc -3' miRNA: 3'- -CCGUUG-GCAGGGGG----------UCAGC-GGGUUGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 30352 | 0.68 | 0.469132 |
Target: 5'- gGGCGACCGUgCCUUGGcccgcaucaUCGCCgAugGu -3' miRNA: 3'- -CCGUUGGCAgGGGGUC---------AGCGGgUugC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 54488 | 0.68 | 0.440492 |
Target: 5'- cGGCAGCgGUCCCggggaguugCCGuUCGCCUGAUa -3' miRNA: 3'- -CCGUUGgCAGGG---------GGUcAGCGGGUUGc -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 32458 | 0.68 | 0.437683 |
Target: 5'- cGGCAGCCaugCCCUC-GUCGCCUucgccggcagccucGGCGa -3' miRNA: 3'- -CCGUUGGca-GGGGGuCAGCGGG--------------UUGC- -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 33763 | 0.68 | 0.431168 |
Target: 5'- -aCAGCaCGUCUCCCAGUCaCCgCGGCa -3' miRNA: 3'- ccGUUG-GCAGGGGGUCAGcGG-GUUGc -5' |
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12426 | 3' | -58.8 | NC_003324.1 | + | 9754 | 0.68 | 0.421961 |
Target: 5'- gGGCGuauCCGcCcggCCCCAcGcCGCCCAGCGc -3' miRNA: 3'- -CCGUu--GGCaG---GGGGU-CaGCGGGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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