Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12430 | 5' | -55.5 | NC_003324.1 | + | 44098 | 0.66 | 0.667411 |
Target: 5'- --uCGCAGCGCucggcaugaucccgGCgGCAGCCGAg -3' miRNA: 3'- gcuGCGUCGCGuaua----------UGgCGUCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 39699 | 0.66 | 0.660742 |
Target: 5'- cCGcACGUcGCGcCAUGUGCCcggccugcgguucuGCAGCCAc -3' miRNA: 3'- -GC-UGCGuCGC-GUAUAUGG--------------CGUCGGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 14461 | 0.66 | 0.652948 |
Target: 5'- gGuuGCGGCGCGUugauugGCaGCAGCCGu -3' miRNA: 3'- gCugCGUCGCGUAua----UGgCGUCGGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 50514 | 0.66 | 0.652948 |
Target: 5'- uGAaaCAGCGCug--GCaCGCAGCCAGc -3' miRNA: 3'- gCUgcGUCGCGuauaUG-GCGUCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 39801 | 0.66 | 0.641797 |
Target: 5'- uGugGUGGCuGCAga-ACCGCAGgCCGGg -3' miRNA: 3'- gCugCGUCG-CGUauaUGGCGUC-GGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 152 | 0.66 | 0.641797 |
Target: 5'- uCGACGgcCGGCGCAUAagagcgugGCUGCGGCa-- -3' miRNA: 3'- -GCUGC--GUCGCGUAUa-------UGGCGUCGguu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 36133 | 0.66 | 0.641797 |
Target: 5'- cCGAUaCGGCGUugGUAgUGCGGCCAAg -3' miRNA: 3'- -GCUGcGUCGCGuaUAUgGCGUCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 56017 | 0.66 | 0.641797 |
Target: 5'- gGGCGgAGCGC----GCCGUGGUCAAg -3' miRNA: 3'- gCUGCgUCGCGuauaUGGCGUCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 36273 | 0.66 | 0.640682 |
Target: 5'- uGAUGUcGCGCAacaucagUAUGCUGCAGCa-- -3' miRNA: 3'- gCUGCGuCGCGU-------AUAUGGCGUCGguu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 37271 | 0.67 | 0.612791 |
Target: 5'- -aGCGCAGC-CGUAccgauuucaagauccUGCCGCGGCgCAAg -3' miRNA: 3'- gcUGCGUCGcGUAU---------------AUGGCGUCG-GUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 24153 | 0.67 | 0.608334 |
Target: 5'- uGGCGCAGCGCAccggcgagaagGUAaagcCCGCcaguGCCGAg -3' miRNA: 3'- gCUGCGUCGCGUa----------UAU----GGCGu---CGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 31180 | 0.67 | 0.597209 |
Target: 5'- cCGAgcaGCAGCGCAU-UGCCGCaaGGaCCGg -3' miRNA: 3'- -GCUg--CGUCGCGUAuAUGGCG--UC-GGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 29958 | 0.67 | 0.586114 |
Target: 5'- gGGCGUccuGCGUAgcgGCgGCAGCCGGg -3' miRNA: 3'- gCUGCGu--CGCGUauaUGgCGUCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 54000 | 0.67 | 0.586114 |
Target: 5'- gCGGCGaAGCGCAcuUGCCGC-GCUGAu -3' miRNA: 3'- -GCUGCgUCGCGUauAUGGCGuCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 38160 | 0.67 | 0.575059 |
Target: 5'- cCGACGCgcuucgccagaaGGCGCA-GUACCGCGaggaaguccGCCGu -3' miRNA: 3'- -GCUGCG------------UCGCGUaUAUGGCGU---------CGGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 32534 | 0.67 | 0.575059 |
Target: 5'- gCGACG-AGgGCAUGgcUGCCGUuGCCAGc -3' miRNA: 3'- -GCUGCgUCgCGUAU--AUGGCGuCGGUU- -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 32158 | 0.68 | 0.531402 |
Target: 5'- aGGCGCAGCGag---GCCGaagAGCCAu -3' miRNA: 3'- gCUGCGUCGCguauaUGGCg--UCGGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 14601 | 0.68 | 0.520667 |
Target: 5'- gCGGCGCAGgcgguaGCGUGgcgGCCGgAGCUAc -3' miRNA: 3'- -GCUGCGUCg-----CGUAUa--UGGCgUCGGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 30749 | 0.69 | 0.510017 |
Target: 5'- cCGGCGUGGCGCG-AUcCgCGCAGCUAu -3' miRNA: 3'- -GCUGCGUCGCGUaUAuG-GCGUCGGUu -5' |
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12430 | 5' | -55.5 | NC_003324.1 | + | 3245 | 0.69 | 0.478647 |
Target: 5'- uCGGCGCGGUGCcgGUGCUuucggaGCucGCCAAg -3' miRNA: 3'- -GCUGCGUCGCGuaUAUGG------CGu-CGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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