Results 21 - 38 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12438 | 5' | -54.9 | NC_003324.1 | + | 28335 | 0.66 | 0.78717 |
Target: 5'- uUCCacCGAUC-CGGCAccagucgcagcaguCGAUGaCGCGGc -3' miRNA: 3'- -AGGa-GCUAGaGCCGUu-------------GCUAC-GCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 29207 | 0.68 | 0.657895 |
Target: 5'- cUUCUCG-UC-CGGCAgcacuACGGUGCGCu- -3' miRNA: 3'- -AGGAGCuAGaGCCGU-----UGCUACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 29729 | 0.68 | 0.657895 |
Target: 5'- -aCUCGAUCccggcucaggCGGCGGCGuUGCGCc- -3' miRNA: 3'- agGAGCUAGa---------GCCGUUGCuACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 30094 | 0.68 | 0.668723 |
Target: 5'- uUCCUCGAcgccUUCGGuCAGCGAUuGCGaaGGg -3' miRNA: 3'- -AGGAGCUa---GAGCC-GUUGCUA-CGCg-CC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 30250 | 0.66 | 0.792979 |
Target: 5'- aCCgcCGAcaccaUCGGCGAUGAUGCG-GGc -3' miRNA: 3'- aGGa-GCUag---AGCCGUUGCUACGCgCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 31014 | 0.77 | 0.228748 |
Target: 5'- cUCgUCGAUCgcUUGGUAGCGAccgGCGCGGu -3' miRNA: 3'- -AGgAGCUAG--AGCCGUUGCUa--CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 31845 | 0.71 | 0.45766 |
Target: 5'- cUUCUUGGcauUCUCGGCGAUGAucUGCGCc- -3' miRNA: 3'- -AGGAGCU---AGAGCCGUUGCU--ACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 38003 | 0.66 | 0.763391 |
Target: 5'- gCCggagCGGUUgCGGCGcACGggGCGCGu -3' miRNA: 3'- aGGa---GCUAGaGCCGU-UGCuaCGCGCc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 39773 | 0.67 | 0.732621 |
Target: 5'- -gCUCGAugaUCUCGGC-ACGGUGCa--- -3' miRNA: 3'- agGAGCU---AGAGCCGuUGCUACGcgcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 40068 | 0.66 | 0.763391 |
Target: 5'- cCCUCGccacgCUCGGCGcgACGGacuuggagauUGCGCaGGc -3' miRNA: 3'- aGGAGCua---GAGCCGU--UGCU----------ACGCG-CC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 41343 | 0.66 | 0.783266 |
Target: 5'- gCCUCGAaaUCGGCGcUGG-GgGCGGc -3' miRNA: 3'- aGGAGCUagAGCCGUuGCUaCgCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 43651 | 0.69 | 0.581983 |
Target: 5'- cCCUCaa--UCGGCuGACGuUGCGCGGc -3' miRNA: 3'- aGGAGcuagAGCCG-UUGCuACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 44287 | 0.71 | 0.45766 |
Target: 5'- gCCUCGAUCUgucucgacgaacCGGgcGCGGUgGCGCGGc -3' miRNA: 3'- aGGAGCUAGA------------GCCguUGCUA-CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 44505 | 0.68 | 0.645957 |
Target: 5'- uUCC-CGAUCggaCGGCAcgcgcauGCGGUGCGUc- -3' miRNA: 3'- -AGGaGCUAGa--GCCGU-------UGCUACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 51988 | 0.7 | 0.51835 |
Target: 5'- aUCUCGAUCUCGuCGAUguucugcuuGAUGaCGCGGa -3' miRNA: 3'- aGGAGCUAGAGCcGUUG---------CUAC-GCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 54434 | 0.68 | 0.679518 |
Target: 5'- gCCUCGA-CUgggucauagcCGGCGGCGAga-GCGGa -3' miRNA: 3'- aGGAGCUaGA----------GCCGUUGCUacgCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 57037 | 0.7 | 0.5393 |
Target: 5'- -gCUgGGgagCGGCGGCGAggGCGCGGa -3' miRNA: 3'- agGAgCUagaGCCGUUGCUa-CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 57398 | 0.66 | 0.782286 |
Target: 5'- gUCCUCacugcugGAgggCUCGGCGgagGCGA-GgGCGGc -3' miRNA: 3'- -AGGAG-------CUa--GAGCCGU---UGCUaCgCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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